RRC ID 11786
Author Cho KA, Ju SY, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
Title Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow.
Journal Cell Biol Int
Abstract We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)-expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver-injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co-cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions.
Volume 33(7)
Pages 772-7
Published 2009-7-1
DOI 10.1016/j.cellbi.2009.04.023
PII S1065-6995(09)00105-X
PMID 19427913
MeSH Animals Bone Marrow Cells / cytology* Carbon Tetrachloride / toxicity Chemical and Drug Induced Liver Injury Coculture Techniques Female Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Hepatocytes / cytology Liver Diseases / therapy* Liver Regeneration / physiology* Mesenchymal Stem Cells / cytology* Mice Mice, Inbred C57BL Mice, Transgenic
IF 2.127
Times Cited 73
Mice C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb