RRC ID |
11786
|
著者 |
Cho KA, Ju SY, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
|
タイトル |
Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow.
|
ジャーナル |
Cell Biol Int
|
Abstract |
We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)-expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver-injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co-cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions.
|
巻・号 |
33(7)
|
ページ |
772-7
|
公開日 |
2009-7-1
|
DOI |
10.1016/j.cellbi.2009.04.023
|
PII |
S1065-6995(09)00105-X
|
PMID |
19427913
|
MeSH |
Animals
Bone Marrow Cells / cytology*
Carbon Tetrachloride / toxicity
Chemical and Drug Induced Liver Injury
Coculture Techniques
Female
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Hepatocytes / cytology
Liver Diseases / therapy*
Liver Regeneration / physiology*
Mesenchymal Stem Cells / cytology*
Mice
Mice, Inbred C57BL
Mice, Transgenic
|
IF |
2.571
|
引用数 |
73
|
WOS 分野
|
CELL BIOLOGY
|
リソース情報 |
実験動物マウス |
RBRC00267 |