論文 - 詳細
| RRC ID | 1180 |
|---|---|
| 著者 | Okuda J, Toyotome T, Kataoka N, Ohno M, Abe H, Shimura Y, Seyedarabi A, Pickersgill R, Sasakawa C. |
| タイトル | Shigella effector IpaH9.8 binds to a splicing factor U2AF(35) to modulate host immune responses. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Shigella effectors injected into the host cell via the type III secretion system are involved in various aspects of infection. Here, we show that one of the effectors, IpaH9.8, plays a role in modulating inflammatory responses to Shigella infection. In murine lung infection model, DeltaipaH9.8 mutant caused more severe inflammatory responses with increased pro-inflammatory cytokine production levels than did wild-type Shigella, which resulted in a 30-fold decrease in bacterial colonization. Binding assays revealed that IpaH9.8 has a specific affinity to U2AF(35), a mammalian splicing factor, which interferes with U2AF(35)-dependent splicing as assayed for IgM pre-mRNA. Reducing the U2AF(35) level in HeLa cells and infecting HeLa cells with wild-type caused a decrease in the expression of the il-8, RANTES, GM-CSF, and il-1beta genes as examined by RT-PCR. The results indicate that IpaH9.8 plays a role in Shigella infection to optimize the host inflammatory responses, thus facilitating bacterial colonization within the host epithelial cells. |
| 巻・号 | 333(2) |
| ページ | 531-9 |
| 公開日 | 2005-7-29 |
| DOI | 10.1016/j.bbrc.2005.05.145 |
| PII | S0006-291X(05)01148-4 |
| PMID | 15950937 |
| MeSH | Animals Antigens, Bacterial / immunology* Antigens, Bacterial / metabolism* Bacterial Proteins / immunology* Bacterial Proteins / metabolism* Dysentery, Bacillary / immunology* Dysentery, Bacillary / metabolism* HeLa Cells Histocompatibility Antigens Class II / immunology Histocompatibility Antigens Class II / metabolism Humans Immunologic Factors / immunology Immunologic Factors / metabolism Lung / immunology* Lung / metabolism* Mice Mice, Inbred C57BL Nuclear Proteins / immunology* Nuclear Proteins / metabolism* RNA Splicing / immunology Ribonucleoproteins / immunology* Ribonucleoproteins / metabolism* Splicing Factor U2AF |
| IF | 2.985 |
| 引用数 | 83 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | |