RRC ID 11838
Author Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM.
Title Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
Journal Cell Stem Cell
Abstract Wnt signaling is required for development of mesoderm-derived lineages and expression of transcription factors associated with the primitive streak. In a functional screen, we examined the mesoderm-inducing capacity of transcription factors whose expression was Wnt-dependent in differentiating ESCs. In contrast to many inactive factors, we found that mesoderm posterior 1 (Mesp1) promoted mesoderm development independently of Wnt signaling. Transient Mesp1 expression in ESCs promotes changes associated with epithelial-mesenchymal transition (EMT) and induction of Snai1, consistent with a role in gastrulation. Mesp1 expression also restricted the potential fates derived from ESCs, generating mesoderm progenitors with cardiovascular, but not hematopoietic, potential. Thus, in addition to its effects on EMT, Mesp1 may be capable of generating the recently identified multipotent cardiovascular progenitor from ESCs in vitro.
Volume 3(1)
Pages 55-68
Published 2008-7-3
DOI 10.1016/j.stem.2008.04.004
PII S1934-5909(08)00175-6
PMID 18593559
PMC PMC2497439
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / deficiency Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / physiology* Cardiovascular System / cytology* Cell Differentiation / drug effects Doxycycline / pharmacology Embryonic Stem Cells / cytology* Epithelial Cells / cytology* Epithelial Cells / drug effects Epithelial Cells / physiology Gene Expression Regulation, Developmental Mesenchymal Stromal Cells / cytology* Mesenchymal Stromal Cells / drug effects Mesenchymal Stromal Cells / physiology* Mice Mice, Knockout Wnt Proteins / physiology
IF 23.29
Times Cited 116
Mice Mesp1-cre