| RRC ID |
11838
|
| 著者 |
Lindsley RC, Gill JG, Murphy TL, Langer EM, Cai M, Mashayekhi M, Wang W, Niwa N, Nerbonne JM, Kyba M, Murphy KM.
|
| タイトル |
Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.
|
| ジャーナル |
Cell Stem Cell
|
| Abstract |
Wnt signaling is required for development of mesoderm-derived lineages and expression of transcription factors associated with the primitive streak. In a functional screen, we examined the mesoderm-inducing capacity of transcription factors whose expression was Wnt-dependent in differentiating ESCs. In contrast to many inactive factors, we found that mesoderm posterior 1 (Mesp1) promoted mesoderm development independently of Wnt signaling. Transient Mesp1 expression in ESCs promotes changes associated with epithelial-mesenchymal transition (EMT) and induction of Snai1, consistent with a role in gastrulation. Mesp1 expression also restricted the potential fates derived from ESCs, generating mesoderm progenitors with cardiovascular, but not hematopoietic, potential. Thus, in addition to its effects on EMT, Mesp1 may be capable of generating the recently identified multipotent cardiovascular progenitor from ESCs in vitro.
|
| 巻・号 |
3(1)
|
| ページ |
55-68
|
| 公開日 |
2008-7-3
|
| DOI |
10.1016/j.stem.2008.04.004
|
| PII |
S1934-5909(08)00175-6
|
| PMID |
18593559
|
| PMC |
PMC2497439
|
| MeSH |
Animals
Basic Helix-Loop-Helix Transcription Factors / deficiency
Basic Helix-Loop-Helix Transcription Factors / genetics
Basic Helix-Loop-Helix Transcription Factors / physiology*
Cardiovascular System / cytology*
Cell Differentiation / drug effects
Doxycycline / pharmacology
Embryonic Stem Cells / cytology*
Epithelial Cells / cytology*
Epithelial Cells / drug effects
Epithelial Cells / physiology
Gene Expression Regulation, Developmental
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / physiology*
Mice
Mice, Knockout
Wnt Proteins / physiology
|
| IF |
20.86
|
| 引用数 |
139
|
|
WOS 分野
|
CELL BIOLOGY
CELL & TISSUE ENGINEERING
|
| リソース情報 |
| 実験動物マウス |
RBRC01145 |
