| RRC ID |
11844
|
| 著者 |
Warzée B, Mesnil C, Hober D, Caloone D, Pirottin D, Garigliany MM, Desmecht D, Drion PV, Journot L, Lekeux P, Bureau F, Desmet CJ.
|
| タイトル |
Viral induction of Zac1b through TLR3- and IRF3-dependent pathways.
|
| ジャーナル |
Mol Immunol
|
| Abstract |
Zinc finger protein regulator of apoptosis and cell cycle arrest (Zac1) is a transcription factor able to induce apoptosis or cell cycle arrest through independent pathways. In spite of the important potential functions attributed to Zac1, little is known of its physiological regulation and biological function. We discovered that variant Zac1b was expressed in murine embryonic fibroblasts (MEFs) treated with polyriboinosinic polyribocytidylic acid [poly(I:C)], a synthetic double-stranded RNA. This regulation occurred mainly through Toll-Like Receptor 3 (TLR3)- and Interferon Regulatory Factor 3 (IRF3)-dependent pathways. As TLR3 and IRF3 are central activators of antiviral immunity, we hypothesized that Zac1 may be implicated in antiviral responses. In line with this notion, we observed that Zac1b was expressed in MEFs infected with Encephalomyocarditis virus (EMCV). We also observed that Zac1-deficient MEFs were less sensitive to EMCV-induced cell death than wild-type MEFs. However, Zac1 gene inactivation had no effect on the survival of mice infected with EMCV. In conclusion, this study describes for the first time a transcriptional regulation of Zac1b, induced by synthetic dsRNA and RNA viruses, the functional significance of which remains to be further investigated.
|
| 巻・号 |
48(1-3)
|
| ページ |
119-27
|
| 公開日 |
2010-1-1
|
| DOI |
10.1016/j.molimm.2010.09.004
|
| PII |
S0161-5890(10)00565-1
|
| PMID |
20947170
|
| MeSH |
Animals
Cardiovirus Infections / immunology*
Cell Cycle Proteins / biosynthesis*
Cell Cycle Proteins / genetics
Cell Cycle Proteins / immunology
Encephalomyocarditis virus
Gene Expression
Gene Expression Regulation / immunology*
Genes, Tumor Suppressor
Immunoblotting
In Situ Nick-End Labeling
Interferon Regulatory Factor-3 / immunology*
Interferon Regulatory Factor-3 / metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
RNA Viruses / immunology
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / immunology*
Toll-Like Receptor 3 / immunology*
Toll-Like Receptor 3 / metabolism
Transcription Factors / biosynthesis*
Transcription Factors / genetics
Transcription Factors / immunology
Transfection
|
| IF |
3.641
|
| 引用数 |
2
|
|
WOS 分野
|
IMMUNOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00858 |
