論文 - 詳細
| RRC ID | 11846 |
|---|---|
| 著者 | Kemball CC, Alirezaei M, Flynn CT, Wood MR, Harkins S, Kiosses WB, Whitton JL. |
| タイトル | Coxsackievirus infection induces autophagy-like vesicles and megaphagosomes in pancreatic acinar cells in vivo. |
| ジャーナル | J Virol |
| Abstract |
Autophagy can play an important part in protecting host cells during virus infection, and several viruses have developed strategies by which to evade or even exploit this homeostatic pathway. Tissue culture studies have shown that poliovirus, an enterovirus, modulates autophagy. Herein, we report on in vivo studies that evaluate the effects on autophagy of coxsackievirus B3 (CVB3). We show that in pancreatic acinar cells, CVB3 induces the formation of abundant small autophagy-like vesicles and permits amphisome formation. However, the virus markedly, albeit incompletely, limits the fusion of autophagosomes (and/or amphisomes) with lysosomes, and, perhaps as a result, very large autophagy-related structures are formed within infected cells; we term these structures megaphagosomes. Ultrastructural analyses confirmed that double-membraned autophagy-like vesicles were present in infected pancreatic tissue and that the megaphagosomes were related to the autophagy pathway; they also revealed a highly organized lattice, the individual components of which are of a size consistent with CVB RNA polymerase; we suggest that this may represent a coxsackievirus replication complex. Thus, these in vivo studies demonstrate that CVB3 infection dramatically modifies autophagy in infected pancreatic acinar cells. |
| 巻・号 | 84(23) |
| ページ | 12110-24 |
| 公開日 | 2010-12-1 |
| DOI | 10.1128/JVI.01417-10 |
| PII | JVI.01417-10 |
| PMID | 20861268 |
| PMC | PMC2976412 |
| MeSH | Analysis of Variance Animals Autophagy / physiology* Blotting, Western Coxsackievirus Infections / physiopathology* Enterovirus B, Human* Green Fluorescent Proteins / metabolism HeLa Cells Humans Male Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Confocal Microscopy, Electron, Transmission Microscopy, Immunoelectron Pancreas / cytology* Pancreas / virology Phagosomes / ultrastructure Phagosomes / virology* |
| IF | 4.501 |
| 引用数 | 107 |
| WOS 分野 | VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |