RRC ID 11847
Author Kataoka K, Nishiguchi KM, Kaneko H, van Rooijen N, Kachi S, Terasaki H.
Title The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization.
Journal Invest Ophthalmol Vis Sci
Abstract PURPOSE:To analyze the roles of vitreal macrophages and circulating leukocytes in retinal vascular growth.
METHODS:Bone marrow (BM) cells from green fluorescent protein (GFP) transgenic mice were transplanted into postnatal day (P)1 mice after irradiation. The mice were exposed to 76% to 78% oxygen (P7-P12), to initiate oxygen-induced retinopathy (OIR). The eyes were collected at P8, P17, and P30, to analyze the engraftment of GFP-positive cells in the retina. GFP-positive peritoneal macrophages, clodronate liposomes, or control liposomes were injected into the eyes at P5 or P12 to examine the effects at P8 or P17. The number of Iba1-positive vitreal macrophages was quantified from histologic sections at P12 and P17.
RESULTS:Few transplanted GFP-positive cells were found in the retina at P8 in both wild-type and OIR mice. However, their number increased at P17 during retinal neovascularization in OIR. Most GFP-positive cells were Iba1-positive microglia, which comprised a minority of the total retinal microglia. Intravitreal injection of peritoneal macrophages showed only incidental migration of these cells into the wild-type retinas (P8), whereas the engraftment was more robust, typically around the neovascularization, in OIR mice (P17). Furthermore, native macrophages in the vitreous cavity became fewer (37.7% reduction) during neovascularization in OIR at P17. The selective depletion of vitreal macrophages by clodronate liposomes at P12 reduced retinal neovascularization in OIR mice by 59.0% at P17.
CONCLUSIONS:Vitreal macrophages are attracted to the site of pathologic angiogenesis triggered by retinal ischemia, where they actively participate in vascular development.
Volume 52(3)
Pages 1431-8
Published 2011-3-14
DOI 10.1167/iovs.10-5798
PII iovs.10-5798
PMID 21051720
MeSH Animals Bone Marrow Transplantation Calcium-Binding Proteins / metabolism Cell Movement / physiology Clodronic Acid / toxicity Disease Models, Animal* Green Fluorescent Proteins / genetics Intravitreal Injections Leukocytes / physiology* Lymphocyte Depletion Macrophages, Peritoneal / drug effects Macrophages, Peritoneal / physiology* Mice Mice, Inbred C57BL Mice, Transgenic Microfilament Proteins Microglia / metabolism Oxygen / toxicity Phagocytosis / physiology Retinal Neovascularization / metabolism* Retinal Neovascularization / pathology Retinal Vessels / metabolism* Retinal Vessels / pathology Vitreous Body / cytology*
IF 3.47
Times Cited 38
Mice RBRC00267