論文 - 詳細
| RRC ID | 1186 |
|---|---|
| 著者 | Yamauchi Y, Abe-Dohmae S, Yokoyama S. |
| タイトル | Differential regulation of apolipoprotein A-I/ATP binding cassette transporter A1-mediated cholesterol and phospholipid release. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
We compared apolipoprotein A-I (apoA-I)-mediated release of cellular cholesterol and phospholipid among several fibroblast cell lines. ApoA-I induced phospholipid release from WI-38, MRC-5, BALB/3T3, L929 and CHO-K1, but not from COS-7, while cholesterol was released only from WI-38, MRC-5 and BALB/3T3 without correlation to the cellular cholesterol content. The reaction consequently generated cholesterol-rich high density lipoprotein (HDL) with WI-38, MRC-5 and BALB/3T3, cholesterol-poor HDL with L929 and CHO-K1, and no HDL from COS-7. In contrast, diffusion-mediated cholesterol efflux to cyclodextrin took place with all the cell lines tested in proportion to the cellular free cholesterol content. While caveolin-1 was expressed in all of these cell lines, ATP-binding cassette transporter (ABC) A1 was detected in all but COS-7. We concluded that (1) fibroblasts were categorized into three groups with respect to the interaction with apoA-I, (i) those to produce cholesterol-rich HDL, (ii) cholesterol-poor HDL and (iii) no HDL; (2) ABCAl was required for the assembly of HDL from phospholipid among the cell lines tested; (3) caveolin-1 alone did not induce cholesterol incorporation into the HDL generated. |
| 巻・号 | 1585(1) |
| ページ | 1-10 |
| 公開日 | 2002-11-8 |
| DOI | 10.1016/s1388-1981(02)00304-9 |
| PII | S1388198102003049 |
| PMID | 12457709 |
| MeSH | 2-Hydroxypropyl-beta-cyclodextrin 3T3 Cells ATP Binding Cassette Transporter 1 ATP-Binding Cassette Transporters / metabolism* Animals Apolipoprotein A-I / metabolism* CHO Cells COS Cells Caveolin 1 Caveolins / metabolism Cholesterol / metabolism* Cholesterol, HDL / metabolism Cricetinae Cyclodextrins / metabolism Fibroblasts / metabolism Humans Mice Phospholipids / metabolism* beta-Cyclodextrins* |
| IF | 3.411 |
| 引用数 | 31 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | WI-38 BALB/3T3 clone A31(RCB0005) CHO-K1(RCB0285) COS-7(RCB0539) |