RRC ID 11881
Author Murakami E, Nagata M, Shimoda Y, Kucho K, Higashi S, Abe M, Hashimoto M, Uchiumi T.
Title Nitric oxide production induced in roots of Lotus japonicus by lipopolysaccharide from Mesorhizobium loti.
Journal Plant Cell Physiol.
Abstract Lipopolysaccharide (LPS) is a bacterial molecule that induces nitric oxide (NO) production and triggers defense systems in plant-pathogen interactions. NO production is induced in the roots of Lotus japonicus after inoculation of the roots with its microsymbiont Mesorhizobium loti. However, the rhizobial molecule that induces NO production has not yet been identified. We investigated NO production in the roots of L. japonicus by treatment with LPS of M. loti. LPS was prepared by phenol-hot water extraction and separated into several fractions: polysaccharide, lipooligosaccharide, oligosaccharide and lipid A. In the roots of L. japonicus, NO production was observed with an NO-specific fluorescent dye 4, 10 and 24 h after treatment with each fraction of LPS. NO production was detected 4 h after treatment with all fractions. NO production was also detectable 24 h after treatment, except after treatment with the polysaccharide and oligosaccharide fractions. Expression of a class 1 hemoglobin gene and application of an NO scavenger showed that the treatment with LPS and LOS induced a similar response to inoculation with M. loti. These data suggest that LPS of M. loti induces NO production after inoculation with M. loti.
Volume 52(4)
Pages 610-7
Published 2011-4
DOI 10.1093/pcp/pcr020
PII pcr020
PMID 21330297
MeSH Benzoates / pharmacology Free Radical Scavengers / pharmacology Gene Expression Regulation, Plant Hemoglobins / genetics Hemoglobins / metabolism Imidazoles / pharmacology Lipopolysaccharides / isolation & purification Lipopolysaccharides / metabolism* Loteae / genetics Loteae / metabolism* Loteae / microbiology Mesorhizobium / metabolism* Nitric Oxide / metabolism* Plant Roots / genetics Plant Roots / metabolism Plant Roots / microbiology Reverse Transcriptase Polymerase Chain Reaction Symbiosis Time Factors
IF 4.059
Times Cited 11
Lotus / Glycine Miyakojima MG-20