RRC ID 11883
Author Himuro Y, Tanaka H, Hashiguchi M, Ichikawa T, Nakazawa M, Seki M, Fujita M, Shinozaki K, Matsui M, Akashi R, Hoffmann F.
Title FOX-superroots of Lotus corniculatus, overexpressing Arabidopsis full-length cDNA, show stable variations in morphological traits.
Journal J. Plant Physiol.
Abstract Using the full-length cDNA overexpressor (FOX) gene-hunting system, we have generated 130 Arabidopsis FOX-superroot lines in bird's-foot trefoil (Lotus corniculatus) for the systematic functional analysis of genes expressed in roots and for the selection of induced mutants with interesting root growth characteristics. We used the Arabidopsis-FOX Agrobacterium library (constructed by ligating pBIG2113SF) for the Agrobacterium-mediated transformation of superroots (SR) and the subsequent selection of gain-of-function mutants with ectopically expressed Arabidopsis genes. The original superroot culture of L. corniculatus is a unique host system displaying fast root growth in vitro, allowing continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely hormone-free culture conditions. Several of the Arabidopsis FOX-superroot lines show interesting deviations from normal growth and morphology of roots from SR-plants, such as differences in pigmentation, growth rate, length or diameter. Some of these mutations are of potential agricultural interest. Genomic PCR analysis revealed that 100 (76.9%) out of the 130 transgenic lines showed the amplification of single fragments. Sequence analysis of the PCR fragments from these 100 lines identified full-length cDNA in 74 of them. Forty-three out of 74 full-length cDNA carried known genes. The Arabidopsis FOX-superroot lines of L. corniculatus, produced in this study, expand the FOX hunting system and provide a new tool for the genetic analysis and control of root growth in a leguminous forage plant.
Volume 168(2)
Pages 181-7
Published 2011-1-15
DOI 10.1016/j.jplph.2010.10.003
PII S0176-1617(10)00482-7
PMID 21106274
MeSH Arabidopsis / genetics* DNA, Complementary / genetics Loteae / genetics* Loteae / growth & development* Plant Roots / genetics* Plant Roots / growth & development* Plants, Genetically Modified / genetics* Plants, Genetically Modified / growth & development* Polymerase Chain Reaction Rhizobium / genetics
IF 2.833
Times Cited 4
WOS Category PLANT SCIENCES
Resource
Lotus / Glycine Super Root