論文 - 詳細
| RRC ID | 11883 |
|---|---|
| 著者 | Himuro Y, Tanaka H, Hashiguchi M, Ichikawa T, Nakazawa M, Seki M, Fujita M, Shinozaki K, Matsui M, Akashi R, Hoffmann F. |
| タイトル | FOX-superroots of Lotus corniculatus, overexpressing Arabidopsis full-length cDNA, show stable variations in morphological traits. |
| ジャーナル | J Plant Physiol |
| Abstract |
Using the full-length cDNA overexpressor (FOX) gene-hunting system, we have generated 130 Arabidopsis FOX-superroot lines in bird's-foot trefoil (Lotus corniculatus) for the systematic functional analysis of genes expressed in roots and for the selection of induced mutants with interesting root growth characteristics. We used the Arabidopsis-FOX Agrobacterium library (constructed by ligating pBIG2113SF) for the Agrobacterium-mediated transformation of superroots (SR) and the subsequent selection of gain-of-function mutants with ectopically expressed Arabidopsis genes. The original superroot culture of L. corniculatus is a unique host system displaying fast root growth in vitro, allowing continuous root cloning, direct somatic embryogenesis and mass regeneration of plants under entirely hormone-free culture conditions. Several of the Arabidopsis FOX-superroot lines show interesting deviations from normal growth and morphology of roots from SR-plants, such as differences in pigmentation, growth rate, length or diameter. Some of these mutations are of potential agricultural interest. Genomic PCR analysis revealed that 100 (76.9%) out of the 130 transgenic lines showed the amplification of single fragments. Sequence analysis of the PCR fragments from these 100 lines identified full-length cDNA in 74 of them. Forty-three out of 74 full-length cDNA carried known genes. The Arabidopsis FOX-superroot lines of L. corniculatus, produced in this study, expand the FOX hunting system and provide a new tool for the genetic analysis and control of root growth in a leguminous forage plant. |
| 巻・号 | 168(2) |
| ページ | 181-7 |
| 公開日 | 2011-1-15 |
| DOI | 10.1016/j.jplph.2010.10.003 |
| PII | S0176-1617(10)00482-7 |
| PMID | 21106274 |
| MeSH | Arabidopsis / genetics* DNA, Complementary / genetics Lotus / genetics* Lotus / growth & development* Plant Roots / genetics* Plant Roots / growth & development* Plants, Genetically Modified / genetics* Plants, Genetically Modified / growth & development* Polymerase Chain Reaction Rhizobium / genetics |
| IF | 3.013 |
| 引用数 | 9 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Super Root |