RRC ID 119
Author Tokuda S, Kuramoto T, Serikawa T.
Title PCR-based genotyping of the rat Atrn(mv) mutation.
Journal Exp. Anim.
Abstract Rat myelin vacuolation mutation at the Attractin locus (Atrn(mv)) is a genomic deletion including the whole exon 1 of the Atrn gene. The precise size and location of the deleted region has not yet been identified because of poor information on genomic organization of the rat Atrn gene. Here, we identified the breakpoints of the Atrn(mv) mutation, using a draft sequence of the rat genome. In the Atrn(mv/mv) rat, a 6,914-bp genomic region was deleted. Primers flanked 5'- and 3'- breakpoints amplified the Atrn(mv) allele but not the wild-type allele. This primer set enables us to distinguish Atrn(mv/+) heterozygous rats from Atrn(+/+) rats, and will contribute to the efficient production of Atrn(mv/mv) rats.
Volume 53(1)
Pages 73-6
Published 2004-1
PMID 14993747
MeSH Animals Base Sequence DNA Primers Electrophoresis Exons / genetics Gene Deletion* Genome* Membrane Proteins / genetics* Molecular Sequence Data Polymerase Chain Reaction Rats / genetics* Sequence Tagged Sites
IF 1.173
Times Cited 5
Rats WTC.ZI-Atrnzi/Kyo(strainID=22)