RRC ID 11906
Author Yamamizo C, Kishimoto S, Ohmiya A.
Title Carotenoid composition and carotenogenic gene expression during Ipomoea petal development.
Journal J. Exp. Bot.
Abstract Japanese morning glory (Ipomoea nil) is a representative plant lacking a yellow-flowered cultivar, although a few wild Ipomoea species contain carotenoids in their petals such as Ipomoea sp. (yellow petals) and I. obscura (pale-yellow petals). In the present study, carotenoid composition and the expression patterns of carotenogenic genes during petal development were compared among I. nil, I. obscura, and Ipomoea sp. to identify the factors regulating carotenoid accumulation in Ipomoea plant petals. In the early stage, the carotenoid composition in petals of all the Ipomoea plants tested was the same as in the leaves mainly showing lutein, violaxanthin, and beta-carotene (chloroplast-type carotenoids). However, in fully opened flowers, chloroplast-type carotenoids were entirely absent in I. nil, whereas they were present in trace amounts in the free form in I. obscura. At the late stage of petal development in Ipomoea sp., the majority of carotenoids were beta-cryptoxanthin, zeaxanthin, and beta-carotene (chromoplast-type carotenoids). In addition, most of them were present in the esterified form. Carotenogenic gene expression was notably lower in I. nil than in Ipomoea sp. In particular, beta-ring hydroxylase (CHYB) was considerably suppressed in petals of both I. nil and I. obscura. The CHYB expression was found to be significantly high in the petals of Ipomoea sp. during the synthesis of chromoplast-type carotenoids. The expression levels of carotenoid cleavage genes (CCD1 and CCD4) were not correlated with the amount of carotenoids in petals. These results suggest that both I. obscura and I. nil lack the ability to synthesize chromoplast-type carotenoids because of the transcriptional down-regulation of carotenogenic genes. CHYB, an enzyme that catalyses the addition of a hydroxyl residue required for esterification, was found to be a key enzyme for the accumulation of chromoplast-type carotenoids in petals.
Volume 61(3)
Pages 709-19
Published 2010-3
DOI 10.1093/jxb/erp335
PII erp335
PMID 19933319
PMC PMC2814104
MeSH Carotenoids / biosynthesis Carotenoids / genetics* Carotenoids / metabolism* Flowers / genetics* Flowers / growth & development* Gene Expression Regulation, Developmental Gene Expression Regulation, Plant* Genes, Plant / genetics Ipomoea nil / genetics* Ipomoea nil / growth & development* Ipomoea nil / metabolism Models, Biological Molecular Sequence Data Plant Leaves / metabolism
IF 5.354
Times Cited 43
Morning Glory