論文 - 詳細
| RRC ID | 11909 |
|---|---|
| 著者 | Kawashima H. |
| タイトル | Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues. |
| ジャーナル | Methods Enzymol |
| Abstract |
Recent studies using sulfotransferase-deficient mice have revealed various physiological functions of sulfated glycans. Studies using gene-targeted mice deficient in both N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST)-1 and GlcNAc6ST-2 showed that these sulfotransferases play critical roles in lymphocyte homing. Recent studies indicated that GlcNAc6ST-2 is expressed not only in lymph node high endothelial venules but also in the colonic epithelial cells in mice, and that this sulfotransferase plays a critical role in GlcNAc-6-O-sulfation of the colonic-mucins, as revealed by liquid chromatography coupled to electrospray ionization tandem mass spectrometry of the colonic-mucin O-glycans from wild-type (WT) and GlcNAc6ST-2-deficient mice. After induction of colitis by dextran sulfate sodium, significantly more leukocyte infiltration was observed in the colon of GlcNAc6ST-2-deficient mice than in that of WT mice. These studies demonstrate that GlcNAc-6-O-sulfotransferases play important roles not only in lymphoid tissues but also in nonlymphoid tissues. This chapter describes experimental procedures for assessing the functions of GlcNAc-6-O-sulfotransferases using gene-targeted mice. |
| 巻・号 | 479 |
| ページ | 243-56 |
| 公開日 | 2010-1-1 |
| DOI | 10.1016/S0076-6879(10)79014-5 |
| PII | S0076-6879(10)79014-5 |
| PMID | 20816170 |
| MeSH | Acetylglucosamine / analogs & derivatives Acetylglucosamine / metabolism Animals Colon / cytology Colon / enzymology Epithelial Cells / enzymology Gene Targeting Immunohistochemistry Lymphoid Tissue / enzymology* Mice Mice, Knockout Receptors, Lymphocyte Homing / metabolism Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Mass, Electrospray Ionization Sulfotransferases / metabolism* |
| IF | 1.394 |
| 引用数 | 0 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 実験動物マウス | RBRC01361 |