| RRC ID |
11936
|
| Author |
Oba M, Miyata K, Osada K, Christie RJ, Sanjoh M, Li W, Fukushima S, Ishii T, Kano MR, Nishiyama N, Koyama H, Kataoka K.
|
| Title |
Polyplex micelles prepared from ω-cholesteryl PEG-polycation block copolymers for systemic gene delivery.
|
| Journal |
Biomaterials
|
| Abstract |
Polyplex micelles formed with plasmid DNA (pDNA) and poly(ethylene glycol) (PEG)-block-poly{N-[N-(2-aminoethyl)-2-aminoethyl]aspartamide} [PAsp(DET)] exhibit effective endosomal escaping properties based on di-protonation of diamine side chains with decreasing pH, which improves their transfection efficiency and thus are promising candidates for local in vivo gene transfer. Here, PEG-PAsp(DET) polyplex micelles were further improved as in vivo systemic vectors by introduction of cholesterol (Chole) into the ω-terminus of PEG-PAsp(DET) to obtain PEG-PAsp(DET)-Chole. Introduction of the cholesterol resulted in enhanced association of block copolymers with pDNA, which led to increased stability in proteinous medium and also in the blood stream after systemic injection compared to PEG-PAsp(DET) micelles. The synergistic effect between enhanced polymer association with pDNA and increased micelle stability of PEG-PAsp(DET)-Chole polyplex micelles led to high in vitro gene transfer even at relatively low concentrations, due to efficient cellular uptake and effective endosomal escape of block copolymers and pDNA. Finally, PEG-PAsp(DET)-Chole micelles achieved significant suppression of tumor growth following intravenous injection into mice bearing a subcutaneous pancreatic tumor using therapeutic pDNA encoding an anti-angiogenic protein. These results suggest that PEG-PAsp(DET)-Chole micelles can be effective systemic gene vectors for treatment of solid tumors.
|
| Volume |
32(2)
|
| Pages |
652-63
|
| Published |
2011-1-1
|
| DOI |
10.1016/j.biomaterials.2010.09.022
|
| PII |
S0142-9612(10)01175-0
|
| PMID |
20932567
|
| MeSH |
Animals
Cell Line, Tumor
Female
Gene Transfer Techniques*
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Micelles*
Microscopy, Confocal
Microscopy, Fluorescence
Polyamines / chemistry*
Polyelectrolytes
Polyethylene Glycols / chemistry*
Polymers / chemistry*
Serum Albumin, Bovine / chemistry
Transfection / methods
Ultracentrifugation
|
| IF |
10.317
|
| Times Cited |
78
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| DNA material |
pCAcc-Luc+ (RDB02443) |
