| RRC ID |
11946
|
| Author |
Yuasa K, Uehara S, Nagahama M, Tsuji A.
|
| Title |
Transcriptional regulation of cGMP-dependent protein kinase II (cGK-II) in chondrocytes.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
The C-type natriuretic peptide/natriuretic peptide receptor-B/cGMP pathway plays an important role in the regulation of endochondral ossification. In chondrocytes, the physiological effect of cGMP is mediated primarily by the activation of cGMP-dependent protein kinase II (cGK-II). In this study, we investigated the transcriptional regulation of cGK-II in chondrocytes. The expression pattern of cGK-II transcripts was examined during chondrogenic differentiation of ATDC5 cells. cGK-II mRNA was not detectable in undifferentiated cells, but increased dramatically prior to differentiation to the hypertrophic stage. To analyze the transcriptional regulation of cGK-II, the 5'-flanking region of the mouse cGK-II gene was isolated and characterized. The promoter activity of the cGK-II gene decreased markedly following deletion and mutagenesis of the putative Nkx-binding site between nucleotide positions -292 and -286. These results suggest that the homeobox gene Nkx family is critical for the transcriptional regulation of cGK-II during chondrogenesis.
|
| Volume |
74(1)
|
| Pages |
44-9
|
| Published |
2010-1-1
|
| DOI |
10.1271/bbb.90529
|
| PII |
JST.JSTAGE/bbb/90529
|
| PMID |
20057151
|
| MeSH |
Animals
Base Sequence
Cartilage / cytology
Cartilage / metabolism
Cartilage / physiology
Cell Differentiation / genetics
Cell Line, Tumor
Chondrocytes / metabolism*
Chondrogenesis / genetics
Cyclic GMP-Dependent Protein Kinase Type II
Cyclic GMP-Dependent Protein Kinases / genetics*
Gene Expression Regulation, Enzymologic
Mice
Molecular Sequence Data
Promoter Regions, Genetic / genetics
Rats
Transcription, Genetic*
|
| IF |
1.516
|
| Times Cited |
5
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
MSM Mouse BAC (RDB04214) |
