| RRC ID |
11957
|
| 著者 |
Katsu Y, Taniguchi E, Urushitani H, Miyagawa S, Takase M, Kubokawa K, Tooi O, Oka T, Santo N, Myburgh J, Matsuno A, Iguchi T.
|
| タイトル |
Molecular cloning and characterization of ligand- and species-specificity of amphibian estrogen receptors.
|
| ジャーナル |
Gen Comp Endocrinol
|
| Abstract |
Estrogens are essential for normal reproductive activity in both males and females as well as for ovarian differentiation during a critical developmental stage in most vertebrates. To understand the molecular mechanisms of estrogen action and to evaluate estrogen receptor ligand interactions in amphibians, we isolated cDNAs encoding the estrogen receptors (ERalpha and ERbeta) from the Japanese firebelly newt (Cynops pyrrhogaster), Tokyo salamander (Hynobius tokyoensis), axolotl (Ambystoma mexicanum), and Raucous toad (Bufo rangeri). Full-length amphibian ER cDNAs were obtained using 5' and 3' rapid amplification of cDNA ends. The predicted amino acid sequences of these amphibian ERs showed a high degree of amino acid sequence identity (over 70%) to each other. We analyzed the relationships of these amphibian ER sequences to other vertebrate ER sequences by constructing a phylogenetic tree. We verified that these were bona fide estrogen receptors using receptor dependent reporter gene assays. We analyzed the effects of natural estrogens, ethinylestradiol, and DDT and its metabolites on the transactivation of the four amphibian species listed above, and Xenopus tropicalis ERs and found that there were species-specific differences in the sensitivity of these ERs to hormones and environmental chemicals. These findings will expand our knowledge of endocrine-disrupting events in amphibians.
|
| 巻・号 |
168(2)
|
| ページ |
220-30
|
| 公開日 |
2010-9-1
|
| DOI |
10.1016/j.ygcen.2010.01.002
|
| PII |
S0016-6480(10)00005-5
|
| PMID |
20064516
|
| MeSH |
Ambystoma mexicanum / genetics
Ambystoma mexicanum / metabolism
Amphibian Proteins / classification*
Amphibian Proteins / genetics
Amphibian Proteins / metabolism*
Amphibians / genetics
Amphibians / metabolism*
Animals
Anura / genetics
Anura / metabolism
Cloning, Molecular
Estrogen Receptor alpha / classification
Estrogen Receptor alpha / genetics
Estrogen Receptor alpha / metabolism
Estrogen Receptor beta / classification
Estrogen Receptor beta / genetics
Estrogen Receptor beta / metabolism
Female
Male
Receptors, Estrogen / classification*
Receptors, Estrogen / genetics
Receptors, Estrogen / metabolism*
Salamandridae / genetics
Salamandridae / metabolism
Urodela / genetics
Urodela / metabolism
|
| IF |
2.428
|
| 引用数 |
22
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ツメガエル・イモリ |
|
