RRC ID 11964
著者 Long N, Hardiville S, Pierce A, Fukamachi K, Futakuchi M, Alexander DB, Shirai T, Tsuda H.
タイトル Construction of a multi-functional helper-dependent adenovirus based system for cancer gene therapy.
ジャーナル Asian Pac J Cancer Prev
Abstract Adenovirus holds great promise as a gene delivery system; it can hold large amounts of exogenous DNA and can be chemically and genetically modified to improve targeting to specific cells and tissues. A recombinant adenovirus construct expressing p53 is currently in clinical use as a cancer therapy in China. However, the use of adenovirus constructs in therapy is limited due to patients' strong immune response against these viruses and their gene products. To overcome this problem helper-dependent adenoviruses which do not express any viral gene products have been developed. Because the helper-dependent viruses do not express any viral gene products, a helper virus is required for their replication and encapsidation into infectious particles. This manuscript describes the construction of a prototype helper-dependent adenovirus system built such that it can be easily modified. The helper-dependent virus described here is built of a series of four cassettes, each with its own function. Furthermore, each individual cassette can be removed and replaced with a cassette with a different function. In this way, different helper-dependent viruses can be readily created. This type of system could be very useful in cancer therapy: For example, libraries of different cassettes could be maintained, allowing rapid assembly of constructs able to provide therapy for individual tumor types.
巻・号 10(5)
ページ 939-60
公開日 2009-1-1
PMID 20104994
MeSH Adenoviridae / genetics* Base Sequence Gene Transfer Techniques Genetic Therapy* Genetic Vectors / therapeutic use* Helper Viruses / genetics* Humans Molecular Sequence Data Neoplasms / genetics* Neoplasms / therapy* Plasmids Transduction, Genetic
引用数 1
WOS 分野 ONCOLOGY
リソース情報
遺伝子材料 pxCANCre (RDB1675)