Reference - Detail
| RRC ID | 12023 |
|---|---|
| Author | Teo JW, Ng KY, Lin RT. |
| Title | Detection and genetic characterisation of qnrB in hospital isolates of Klebsiella pneumoniae in Singapore. |
| Journal | Int J Antimicrob Agents |
| Abstract |
Polymerase chain reaction (PCR) screening of 116 ciprofloxacin-resistant Klebsiella pneumoniae hospital isolates for the presence of qnr genes that mediate plasmid quinolone resistance revealed that none were positive for qnrA or qnrS. However, qnrB was detected in ca. 5.2% of the isolates. Southern hybridisation demonstrated that the qnrB-hybridising plasmids were large (>70kb) and capable of transferring quinolone resistance by conjugation. Sequence analysis of the qnrB genes detected in this study showed that they were identical to previously identified qnrB1, qnrB4 and qnrB6 genes, although a novel variant designated qnrB20 was also identified. Analysis of the genetic environment around the cloned qnrB genes showed that they were present in diverse plasmid backbones, sometimes within novel genetic contexts, but always associated with mobile or transposable elements. |
| Volume | 33(2) |
| Pages | 177-80 |
| Published | 2009-2-1 |
| DOI | 10.1016/j.ijantimicag.2008.08.019 |
| PII | S0924-8579(08)00405-6 |
| PMID | 18993034 |
| MeSH | Anti-Bacterial Agents / pharmacology* Bacterial Proteins / genetics* Blotting, Southern Conjugation, Genetic DNA, Bacterial / chemistry DNA, Bacterial / genetics Drug Resistance, Bacterial* Gene Transfer, Horizontal Hospitals Humans Interspersed Repetitive Sequences Klebsiella Infections / microbiology* Klebsiella pneumoniae / drug effects* Klebsiella pneumoniae / genetics Klebsiella pneumoniae / isolation & purification Molecular Sequence Data Plasmids Polymerase Chain Reaction Quinolones / pharmacology* Sequence Analysis, DNA Singapore |
| IF | 4.621 |
| Times Cited | 18 |
| WOS Category | PHARMACOLOGY & PHARMACY INFECTIOUS DISEASES MICROBIOLOGY |
| Resource | |
| DNA material | pBC-H2.6 (RDB05463) |