論文 - 詳細
| RRC ID | 12024 |
|---|---|
| 著者 | Clarke JD, Hsu A, Williams DE, Dashwood RH, Stevens JF, Yamamoto M, Ho E. |
| タイトル | Metabolism and tissue distribution of sulforaphane in Nrf2 knockout and wild-type mice. |
| ジャーナル | Pharm Res |
| Abstract |
PURPOSE:To determine the metabolism and tissue distribution of the dietary chemoprotective agent sulforaphane following oral administration to wild-type and Nrf2 knockout (Nrf2(-/-)) mice. METHODS:Male and female wild-type and Nrf2(-/-) mice were given sulforaphane (5 or 20 μmoles) by oral gavage; plasma, liver, kidney, small intestine, colon, lung, brain and prostate were collected at 2, 6 and 24 h (h). The five major metabolites of sulforaphane were measured in tissues by high performance liquid chromatography coupled with tandem mass spectrometry. RESULTS:Sulforaphane metabolites were detected in all tissues at 2 and 6 h post gavage, with the highest concentrations in the small intestine, prostate, kidney and lung. A dose-dependent increase in sulforaphane concentrations was observed in all tissues except prostate. At 5 μmole, Nrf2(-/-) genotype had no effect on sulforaphane metabolism. Only Nrf2(-/-) females given 20 μmoles sulforaphane for 6 h exhibited a marked increase in tissue sulforaphane metabolite concentrations. The relative abundance of each metabolite was not strikingly different between genders and genotypes. CONCLUSIONS:Sulforaphane is metabolized and reaches target tissues in wild-type and Nrf2(-/-) mice. These data provide further evidence that sulforaphane is bioavailable and may be an effective dietary chemoprevention agent for several tissue sites. |
| 巻・号 | 28(12) |
| ページ | 3171-9 |
| 公開日 | 2011-12-1 |
| DOI | 10.1007/s11095-011-0500-z |
| PMID | 21681606 |
| PMC | PMC3253624 |
| MeSH | Animals Anticarcinogenic Agents / metabolism* Anticarcinogenic Agents / pharmacokinetics* Female Isothiocyanates Male Mice Mice, Knockout NF-E2-Related Factor 2 / genetics* Sulfoxides Thiocyanates / metabolism* Thiocyanates / pharmacokinetics* Tissue Distribution |
| IF | 3.242 |
| 引用数 | 88 |
| WOS 分野 | PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| リソース情報 | |
| 実験動物マウス | RBRC00984 |