論文 - 詳細
| RRC ID | 12030 |
|---|---|
| 著者 | Takehashi M, Kanatsu-Shinohara M, Inoue K, Ogonuki N, Miki H, Toyokuni S, Ogura A, Shinohara T. |
| タイトル | Adenovirus-mediated gene delivery into mouse spermatogonial stem cells. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Spermatogonial stem cells represent a self-renewing population of spermatogonia, and continuous division of these cells supports spermatogenesis throughout the life of adult male animals. Previous attempts to introduce adenovirus vectors into spermatogenic cells, including spermatogonial stem cells, have failed to yield evidence of infection, suggesting that male germ cells may be resistant to adenovirus infection. In this study we show the feasibility of transducing spermatogonial stem cells by adenovirus vectors. When testis cells from ROSA26 Cre reporter mice were incubated in vitro with a Cre-expressing adenovirus vector, Cre-mediated recombination occurred at an efficiency of 49-76%, and the infected spermatogonial stem cells could reinitiate spermatogenesis after transplantation into seminiferous tubules of infertile recipient testes. No evidence of germ-line integration of adenovirus vector could be found in offspring from infected stem cells that underwent Cre-mediated recombination, which suggests that the adenovirus vector infected the cells but did not stably integrate into the germ line. Nevertheless, these results suggest that adenovirus may inadvertently integrate into the patient's germ line and indicate that there is no barrier to adenovirus infection in spermatogonial stem cells. |
| 巻・号 | 104(8) |
| ページ | 2596-601 |
| 公開日 | 2007-2-20 |
| DOI | 10.1073/pnas.0609282104 |
| PII | 0609282104 |
| PMID | 17299052 |
| PMC | PMC1815228 |
| MeSH | Adenoviridae / metabolism* Adenoviridae / physiology Animals Female Gene Deletion Gene Transfer Techniques* Humans Insemination Integrases / metabolism Male Mice Proteins / genetics RNA, Untranslated Recombination, Genetic Spermatogenesis Spermatogonia / cytology* Spermatogonia / metabolism* Spermatogonia / virology Stem Cells / cytology* Stem Cells / metabolism* Stem Cells / virology Virus Integration beta-Galactosidase / metabolism |
| IF | 9.412 |
| 引用数 | 46 |
| WOS 分野 | REPRODUCTIVE BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | AxCANLacZ (RDB03211) AxCANCre (RDB01748). |