| RRC ID |
12031
|
| 著者 |
Kawata S, Suzuki J, Maruoka M, Mizutamari M, Ishida-Kitagawa N, Yogo K, Jat PS, Shishido T.
|
| タイトル |
Retrovirus-mediated conditional immortalization and analysis of established cell lines of osteoclast precursor cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Osteoclast precursor cells (OPCs) have previously been established from bone marrow cells of SV40 temperature-sensitive T antigen-expressing transgenic mice. Here, we use retrovirus-mediated gene transfer to conditionally immortalize OPCs by expressing temperature-sensitive large T antigen (tsLT) from wild type bone marrow cells. The immortalized OPCs proliferated at the permissive temperature of 33.5 degrees C, but stopped growing at the non-permissive temperature of 39 degrees C. In the presence of receptor activator of NFkappaB ligand (RANKL), the OPCs differentiated into tartrate-resistant acid phosphatase (TRAP)-positive cells and formed multinucleate osteoclasts at 33.5 degrees C. From these OPCs, we cloned two types of cell lines. Both differentiated into TRAP-positive cells, but one formed multinucleate osteoclasts while the other remained unfused in the presence of RANKL. These results indicate that the established cell lines are useful for analyzing mechanisms of differentiation, particularly multinucleate osteoclast formation. Retrovirus-mediated conditional immortalization should be a useful method to immortalize OPCs from primary bone marrow cells.
|
| 巻・号 |
350(1)
|
| ページ |
97-104
|
| 公開日 |
2006-11-10
|
| DOI |
10.1016/j.bbrc.2006.09.020
|
| PII |
S0006-291X(06)02020-1
|
| PMID |
17005159
|
| MeSH |
Animals
Cell Differentiation* / drug effects
Cell Survival
Mice
Osteoclasts / cytology*
Osteoclasts / drug effects
Osteoclasts / metabolism
RANK Ligand / pharmacology
Retroviridae / genetics*
Stem Cells / cytology*
Stem Cells / drug effects
Temperature
|
| IF |
2.985
|
| 引用数 |
3
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pCAhMCSF Human M-CSF(RDB01524) |
