| RRC ID |
12058
|
| Author |
Hirota K, Hanyu Y.
|
| Title |
Method for identification of mutant glutathione S-transferases conferring enhanced resistance to the anti-cancer drug chlorambucil.
|
| Journal |
J Biosci Bioeng
|
| Abstract |
We screened library of mutant glutathione S-transferases (GSTs) in Escherichia coli by successive treatments with anti-cancer drug chlorambucil and identified mutant GSTs that conferred enhanced resistance to host against chlorambucil compared with wild-type GST. This study provides a method to develop enzymes with improved efficiency of detoxification against cytotoxic substances.
|
| Volume |
93(6)
|
| Pages |
618-21
|
| Published |
2002-1-1
|
| DOI |
10.1016/s1389-1723(02)80248-1
|
| PII |
S1389-1723(02)80248-1
|
| PMID |
16233260
|
| IF |
2.366
|
| Times Cited |
0
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
FOOD SCIENCE & TECHNOLOGY
|
| Resource |
| DNA material |
pGPi2 Human glutathione transferase-pi cDNA (RDB01393) |
