Reference - Detail
| RRC ID | 12059 |
|---|---|
| Author | Nakamura T, Sasaki T, Fujimori M, Yazawa K, Kano Y, Amano J, Taniguchi S. |
| Title | Cloned cytosine deaminase gene expression of Bifidobacterium longum and application to enzyme/pro-drug therapy of hypoxic solid tumors. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Bifidobacterium longum is a nonpathogenic anaerobic bacterium among normal bacterial flora. Recently, it was reported that B. longum accumulated in hypoxic solid tumors. The gene of interest was expressed in transfected B. longum by the shuttle vector pBLES100 in solid tumors. In this report, we constructed pBLES100-S-eCD, which included the cytosine deaminase gene. We confirmed by western blotting that transfected B. longum produced cytosine deaminase. In addition, transfected B. longum produced cytosine deaminase that converted 5-fluorocytosine into 5-fluorouracil. B. longum could be useful for enzyme/pro-drug therapy of hypoxic solid tumors. |
| Volume | 66(11) |
| Pages | 2362-6 |
| Published | 2002-11-1 |
| DOI | 10.1271/bbb.66.2362 |
| PMID | 12506973 |
| MeSH | Antimetabolites, Antineoplastic / pharmacokinetics* Antimetabolites, Antineoplastic / therapeutic use Bifidobacterium / enzymology* Bifidobacterium / genetics* Bifidobacterium / metabolism Blotting, Western Cloning, Molecular Cytosine Deaminase Flucytosine / pharmacokinetics* Flucytosine / therapeutic use Fluorouracil / pharmacokinetics* Fluorouracil / therapeutic use Gene Expression Genetic Therapy / methods* Genetic Vectors / genetics Hypoxia / physiopathology Neoplasms / drug therapy Nucleoside Deaminases / genetics* Nucleoside Deaminases / metabolism* Prodrugs / pharmacokinetics* Prodrugs / therapeutic use Transfection Transformation, Bacterial |
| IF | 1.516 |
| Times Cited | 49 |
| WOS Category | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 19 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pAxCACD pAdex1CACD (RDB1591) |