RRC ID 12062
Author Fujise H, Annoura T, Sasawatari S, Ikeda T, Ueda K.
Title Transepithelial transport and cellular accumulation of steroid hormones and polychlorobiphenyl in porcine kidney cells expressed with human P-glycoprotein.
Journal Chemosphere
Abstract Endocrine disrupters such as sex hormone-like chemicals and the non-physiological ligands for aryl hydrocarbon receptor (AhR) exert many adverse biological effects. The ligands for AhR disturb gene expression downstream of the gene induced by estrogen receptor at a very low concentration. Thus, transepithelial transport and cellular accumulation of cortisol (COR) and estrogen as congeners of sex hormone-like chemicals, and 3,3',4,4'-tetrachlorobiphenyl (TeCB) as one of the ligands for AhR were examined in a monolayer of porcine kidney cells transfected with human P-glycoprotein (LLC-COL). The net basal-to-apical transport of COR increased in LLC-COL compared to that in the wild type cells (LLC-PKI) the same as in vinblastine, whereas the net transport of estradiol (EST) was not detected in either cell group. Though the diffusion transports of EST for both directions, basal-to-apical and apical-to-basal, were higher than that of COR, cellular accumulation of EST was higher than that of COR. Transepithelial transport of TeCB was very low and the net basal-to-apical transport was not detected, while it was highly accumulated in the epithelial cells. The accumulation was slightly higher in LLC-COL than in LLC-PKI at high dose.
Volume 46(9-10)
Pages 1505-11
Published 2002-3-1
DOI 10.1016/s0045-6535(01)00273-9
PII S0045-6535(01)00273-9
PMID 12002483
MeSH ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis* ATP Binding Cassette Transporter, Subfamily B, Member 1 / pharmacology Animals Cell Culture Techniques Cell Membrane Endocrine System / drug effects Environmental Pollutants / pharmacokinetics* Estradiol / pharmacokinetics* Gene Expression Regulation Hydrocortisone / pharmacokinetics* Kidney / cytology Ligands Polychlorinated Biphenyls / pharmacokinetics* Receptors, Aryl Hydrocarbon / drug effects Receptors, Aryl Hydrocarbon / physiology* Swine
IF 5.778
Times Cited 10
DNA material Human MDR1 cDNA Human P-glycoprotein cDNA (RDB01372)