| RRC ID |
12069
|
| Author |
Kobayashi N, Taguchi T, Noguchi H, Okitsu T, Totsugawa T, Watanabe T, Matsumura T, Fujiwara T, Urata H, Kishimoto N, Hayashi N, Nakaji S, Murakami T, Tanaka N.
|
| Title |
Rapidly functional immobilization of immortalized human hepatocytes using cell adhesive GRGDS peptide-carrying cellulose microspheres.
|
| Journal |
Cell Transplant
|
| Abstract |
With the development of biotechnology, hepatic support by a hybrid artificial liver (HAL) using hepatocytes has been given much attention. Because the availability of human livers is limited, we have established a tightly regulated immortal human hepatocyte cell line, NKNT-3, for developing HAL. Because high-density cell culture allows the compactness of the HAL device and its easy use under emergency circumstances, we have developed cell adhesive GRGDS peptide-containing cellulose microspheres (GRGDS/CMS). The GRGDS/CMS efficiently immobilized NKNT-3 cells within 24 h in a stirred suspension culture. Electron microscopic examinations demonstrated glycogen granules and well-developed endoplasmic reticulum and mitochondria in NKNT-3 cells attached to the GRGDS/CMS. The cells showed ammonia clearance activity, whereas HepG2-transformed human liver cells did not remove the loaded ammonia. An efficient adenoviral delivery of the lacZ reporter gene was performed in GRGDS/CMS-immobilized NKNT-3 cells. In this study we present rapid immobilization of NKNT-3 immortal human hepatocytes using cellulose microspheres carrying GRGDS peptides. These microspheres satisfied immediate preparation of NKNT-3 cells in sufficient quantity and of adequate quality.
|
| Volume |
10(4-5)
|
| Pages |
387-92
|
| Published |
2001-1-1
|
| PMID |
11549059
|
| MeSH |
Ammonia / metabolism
Cell Adhesion / physiology*
Cell Culture Techniques / methods
Cell Line
Cell Size
Cells, Immobilized*
Cellulose / metabolism
Gene Transfer Techniques
Hepatocytes / physiology*
Hepatocytes / ultrastructure
Humans
Liver, Artificial*
Microspheres*
Oligopeptides* / metabolism
Time Factors
|
| IF |
3.341
|
| Times Cited |
11
|
|
WOS Category
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
CELL & TISSUE ENGINEERING
|
| Resource |
| DNA material |
AxCALacZ Adex1CAlacZ (RDB02726) |
