論文 - 詳細
| RRC ID | 12084 |
|---|---|
| 著者 | Jonz MG, Nurse CA. |
| タイトル | Development of oxygen sensing in the gills of zebrafish. |
| ジャーナル | J Exp Biol |
| Abstract |
Previous studies have described the morphology, innervation and O(2)-chemoreceptive properties of neuroepithelial cells (NECs) of the zebrafish gill filaments. The present work describes the ontogenesis of these cells, and the formation of functional O(2)-sensing pathways in developing zebrafish. Confocal immunofluorescence was performed on whole-mount gill preparations using antibodies against serotonin (5-HT) and a zebrafish-derived neuronal marker (zn-12) to identify the appearance and innervation of gill NECs during larval stages. NECs were first expressed in gill filament primordia of larvae at 5 days postfertilization (d.p.f.) and were fully innervated by 7 d.p.f. In vivo ventilation frequency analysis revealed that a behavioural response to hypoxia (11.2+/-2.8 min(-1)) developed in embryos as early as 2 d.p.f., and a significant increase (P<0.05) in the ventilatory response to hypoxia (200.8+/-23.0 min(-1)) coincided with innervation of NECs of the filaments. In addition, exogenous application of quinidine, a blocker of O(2)-sensitive background K(+) channels in NECs, induced hyperventilation in adults in a dose-dependent manner and revealed the development of a quinidine-sensitive ventilatory response in 7 d.p.f. larvae. This study shows that NEC innervation in the gill filaments may account for the development of a functional O(2)-sensing pathway and the hyperventilatory response to hypoxia in zebrafish larvae. At earlier stages, however, O(2)-sensing must occur through another pathway. The possibility that a new type of 5-HT-positive NEC of the gill arches may account for this earlier hypoxic response is discussed. |
| 巻・号 | 208(Pt 8) |
| ページ | 1537-49 |
| 公開日 | 2005-4-1 |
| DOI | 10.1242/jeb.01564 |
| PII | 208/8/1537 |
| PMID | 15802677 |
| MeSH | Analysis of Variance Animals Chemoreceptor Cells / embryology Chemoreceptor Cells / physiology* Dose-Response Relationship, Drug Gills / embryology* Gills / innervation Gills / physiology Microscopy, Fluorescence Neuroepithelial Cells / cytology Neuroepithelial Cells / physiology* Oxygen / physiology* Oxygen Consumption / drug effects Quinidine / pharmacology Zebrafish / embryology* Zebrafish / physiology |
| IF | 3.014 |
| 引用数 | 85 |
| WOS 分野 | BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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