RRC ID 12087
Author Yazawa R, Hirono I, Ohira T, Aoki T.
Title Induction of Japanese flounder tnf promoter activity by lipopolysaccharide in zebrafish embryo.
Journal Mar Biotechnol (NY)
Abstract A recombinant plasmid containing the 2381-bp promoter region of Japanese flounder tumor necrosis factor (TNF) and green fluorescence protein (GFP) was introduced into zebrafish fertilized eggs by microinjection. GFP was expressed in 2 transgenic zebrafish lines in the heart and around the pharynx under unstressed condition. When embryos were exposed to lipopolysaccharide (LPS), GFP was expressed in the whole zebrafish embryonic surface, and Western blot analysis also showed that the level of the expressed GFP protein in zebrafish embryo was higher at an LPS concentration of 140 microg/ml than at LPS concentrations of 0 and 70 microg/ml. Stimulation with either concanavalin A or phorbol myristate acetate induced weak GFP expression, but stimulation with both of them induced strong expression similar to that induced by LPS. GFP expression peaked 1 hour after stimulation, then gradually decreased. These results indicate that transcription regulated by the Japanese flounder TNF promoter could be under the control of the LPS-recognition system in zebrafish embryos.
Volume 7(3)
Pages 231-5
Published 2005-1-1
DOI 10.1007/s10126-004-0403-7
PMID 15864470
MeSH Animals Animals, Genetically Modified Blotting, Western Concanavalin A / pharmacology DNA Primers Electrophoresis, Polyacrylamide Gel Embryo, Nonmammalian / metabolism Flounder / genetics* Gene Expression Regulation / drug effects* Gene Expression Regulation / genetics Gene Transfer Techniques Green Fluorescent Proteins / metabolism Lipopolysaccharides / metabolism Lipopolysaccharides / pharmacology* Plasmids / genetics Promoter Regions, Genetic / genetics* Reverse Transcriptase Polymerase Chain Reaction Tetradecanoylphorbol Acetate / pharmacology Tumor Necrosis Factors / genetics Tumor Necrosis Factors / metabolism* Zebrafish / embryology
IF 2.798
Times Cited 7