論文 - 詳細
| RRC ID | 1212 |
|---|---|
| 著者 | Uchida E, Mizuguchi H, Ishii-Watabe A, Hayakawa T. |
| タイトル | Comparison of the efficiency and safety of non-viral vector-mediated gene transfer into a wide range of human cells. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Non-viral gene transfer into a wide range of human cells was examined in order to clarify the factors that affect the efficiency and safety of non-viral vectors and to optimize the conditions so that high efficiency and low toxicity could be achieved. Six non-viral vectors (Lipofectin, LipofectAMINE PLUS, SuperFect, Effectene, DMRIE-C and DOTAP) were used to transfect a mammalian expression plasmid pCMVbeta into 16 types of human primary cells and cultured cell lines. Transfection efficiency was quantified using a galactosidase assay. Cytotoxic effects were measured by lactate dehydrogenase (LDH) assay and WST-8 assay. In serum-free conditions, LipofectAMINE PLUS, Effectene and SuperFect, on average, transfected DNA more successfully than Lipofectin, DMRIE-C, and DOTAP, although the levels of gene expression with these vectors varied remarkably in different cells. The most effective vector also differed depending on the cell type. Serum was found to inhibit gene transfer and reduce the cytotoxicity of all of these vectors except Effectene. The efficiency and toxicity of the non-viral vectors used depended on the type of vector, the DNA/vector ratio, the type of cell, and the presence of serum. These results provided useful information for the optimization of transfer conditions of these non-viral vectors. |
| 巻・号 | 25(7) |
| ページ | 891-7 |
| 公開日 | 2002-7-1 |
| DOI | 10.1248/bpb.25.891 |
| PMID | 12132664 |
| MeSH | Cell Line Cell Survival / drug effects DNA / genetics* Gene Expression* Genes, Reporter Genetic Vectors* Humans Lipids / chemistry Liposomes / administration & dosage* Liposomes / chemistry Liposomes / toxicity Transfection / methods* beta-Galactosidase / genetics |
| IF | 1.863 |
| 引用数 | 95 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB0806) |