RRC ID 1216
著者 Nakamura K, Yoshikawa N, Yamaguchi Y, Kagota S, Shinozuka K, Kunitomo M.
タイトル Characterization of mouse melanoma cell lines by their mortal malignancy using an experimental metastatic model.
ジャーナル Life Sci
Abstract We characterized the metastatic ability and mortality of four different mouse melanoma cell lines, B16-F0, -F1, -F10 and -BL6. B16-F0 is the parent cell line. B16-F1 was obtained by a one-time selective procedure and B16-F10 by a ten-time selective procedure using Fidler's method. B16-BL6 derived from B16-F10 has much more invasive activity than B16-F10. To investigate the difference in mortal malignancy among B16-F0, -F1, -F10 and -BL6, we examined the survival time of syngeneic C57BL/6Cr mice intravenously inoculated with these cells. As a control, we used the C57BL/6J-embryo mouse fibroblast-like semi-normal cell line. The ability to form lung metastatic nodules in mice gradually increased in the order: B16-F0, -F1, and -F10 (=-BL6). C57BL/6J-embryo cell (1 x 10(5)/mouse)-inoculated mice survived for over 46 days. B16-F0, -F1, -F10 and -BL6 (1 x 10(5)/mouse)-inoculated mice survived 31.4+/-4.4 (7), 25.7+/-2.8 (7), 23.6+/-1.5 (7) and 25.3+/-2.3 (7) days [mean+/-S.D. (number of mice)], respectively. According to the Mann-Whitney test, the B16-F0 inoculated group versus -F1 inoculated group (P<0.05), -F0 inoculated group versus -BL6 inoculated group (P<0.05), and -F0 inoculated group versus -F10 inoculated group (P<0.01) were significantly different, but the B16-F1 group versus -F10 group, -F1 group versus -BL6 group, and -F10 group versus -BL6 group were not. These results suggest that mortal malignancy is not necessarily correlated with lung-colonizing potential and even only one-time selected B16-F0 mouse melanoma cells are useful as an experimental metastatic model in vivo.
巻・号 70(7)
ページ 791-8
公開日 2002-1-4
DOI 10.1016/s0024-3205(01)01454-0
PII S0024-3205(01)01454-0
PMID 11833741
MeSH Animals Body Weight Disease Models, Animal Female Hematologic Tests Lung / pathology Lung Neoplasms / mortality Lung Neoplasms / secondary* Melanoma / mortality Melanoma / secondary* Mice Mice, Inbred C57BL Neoplasm Transplantation / pathology Organ Size Skin Neoplasms / pathology* Specific Pathogen-Free Organisms Survival Rate Tumor Cells, Cultured
IF 3.647
引用数 42
WOS 分野 MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 C57BL/6J-emb(RCB1312)