論文 - 詳細
| RRC ID | 12160 |
|---|---|
| 著者 | Setoguchi K, Misaki Y, Araki Y, Fujio K, Kawahata K, Kitamura T, Yamamoto K. |
| タイトル | Antigen-specific T cells transduced with IL-10 ameliorate experimentally induced arthritis without impairing the systemic immune response to the antigen. |
| ジャーナル | J Immunol |
| Abstract |
For the treatment of rheumatoid arthritis, efficient drug delivery methods to the inflamed joints need to be developed. Because T cells expressing an appropriate autoantigen-specific receptor can migrate to inflamed lesions, it has been reasoned that they can be employed to deliver therapeutic agents. To examine the ability and efficiency of such T cells as a vehicle, we employed an experimentally induced model of arthritis. Splenic T cells from DO11.10 TCR transgenic mice specific for OVA were transduced with murine IL-10. Adoptive transfer of the IL-10-transduced DO11.10 splenocytes ameliorated OVA-induced arthritis despite the presence of around 95% nontransduced cells. Using green fluorescent protein as a marker for selection, the number of transferred cells needed to ameliorate the disease was able to be reduced to 10(4). Preferential accumulation of the transferred T cells was observed in the inflamed joint, and the improvement in the disease was not accompanied by impairment of the systemic immune response to the Ag, suggesting that the transferred T cells exert their anti-inflammatory task locally, mainly in the joints where the Ag exists. In addition, IL-10-transduced DO11.10 T cells ameliorated methylated BSA-induced arthritis when the arthritic joint was coinjected with OVA in addition to methylated BSA. These results suggest that T cells specific for a joint-specific Ag would be useful as a therapeutic vehicle in rheumatoid arthritis for which the arthritic autoantigen is still unknown. |
| 巻・号 | 165(10) |
| ページ | 5980-6 |
| 公開日 | 2000-11-15 |
| DOI | 10.4049/jimmunol.165.10.5980 |
| PMID | 11067961 |
| MeSH | Animals Antigens / administration & dosage Antigens / immunology* Antigens / pharmacology Arthritis, Experimental / genetics Arthritis, Experimental / immunology* Arthritis, Experimental / pathology Arthritis, Experimental / therapy* CD4-Positive T-Lymphocytes / immunology CD4-Positive T-Lymphocytes / metabolism CD4-Positive T-Lymphocytes / transplantation* Cell Movement / genetics Cell Movement / immunology Dose-Response Relationship, Immunologic Epitopes, T-Lymphocyte / genetics* Female Genetic Vectors / immunology Genetic Vectors / metabolism Green Fluorescent Proteins Immunity, Cellular / genetics Injections, Intra-Articular Interleukin-10 / genetics* Joints / immunology Joints / pathology Luminescent Proteins / biosynthesis Lymphocyte Activation / genetics Lymphocyte Count Lymphocyte Transfusion Mice Mice, Inbred BALB C Mice, Transgenic Ovalbumin / administration & dosage Ovalbumin / immunology* Ovalbumin / pharmacology Retroviridae / genetics Retroviridae / immunology Serum Albumin, Bovine / immunology Severity of Illness Index Spleen / cytology Spleen / immunology Spleen / metabolism Spleen / transplantation Transduction, Genetic* |
| IF | 4.886 |
| 引用数 | 35 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | pMFGmIL10 (RDB1446) |