論文 - 詳細
| RRC ID | 12164 |
|---|---|
| 著者 | Furumoto K, Arii S, Yamasaki S, Mizumoto M, Mori A, Inoue N, Isobe N, Imamura M. |
| タイトル | Spleen-derived dendritic cells engineered to enhance interleukin-12 production elicit therapeutic antitumor immune responses. |
| ジャーナル | Int J Cancer |
| Abstract |
The major goal in cancer immunotherapy is the induction of tumor-specific T lymphocytes capable of killing tumor cells. As both dendritic cells (DCs) and interleukin-12 (IL-12) can play immunostimulatory roles in vivo, the use of a combination of these has become a promising approach. In the present study, we used a murine tumor model to examine whether spleen-derived DCs transduced with the IL-12 gene could elicit tumor-specific immune responses. BALB/c mice injected peritumorally with adenovirus-mediated IL-12 gene-transduced antigen-unpulsed DCs inhibited the growth of day 5-established subcutaneous CT26 tumors. Splenocytes from treated mice responded specifically to parental tumor cells and showed increased production of interferon gamma (IFN-gamma) and antitumor cytotoxic T-lymphocyte (CTL) activity. Increased numbers of both CD4(+) and CD8(+) T cells were detected in the treated tumors. The inhibition of tumor growth was significantly greater in mice injected with IL-12 gene-transduced DCs than in those injected with IL-12 gene-transduced fibroblasts or the IL-12 gene-encoding adenovirus itself. Taken together, these results indicate that DCs transduced with the IL-12 gene by a recombinant adenovirus are effective in inducing tumor-specific Th1 and CTL responses that inhibit the growth of established subcutaneous tumors. |
| 巻・号 | 87(5) |
| ページ | 665-72 |
| 公開日 | 2000-9-1 |
| PII | 10.1002/1097-0215(20000901)87:5<665::AID-IJC8>3.0.CO;2-F |
| PMID | 10925360 |
| MeSH | Adenocarcinoma / immunology Adenocarcinoma / pathology Adenocarcinoma / therapy Adenoviridae / genetics Animals CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Cell Division / physiology Colonic Neoplasms / immunology Colonic Neoplasms / pathology Colonic Neoplasms / therapy Dendritic Cells / immunology* Dendritic Cells / physiology Fibroblasts / physiology Humans Immunotherapy, Adoptive* Interferon-gamma / biosynthesis Interferon-gamma / immunology Interleukin-12 / biosynthesis* Interleukin-12 / genetics* Interleukin-12 / immunology Interleukin-4 / biosynthesis Interleukin-4 / immunology Lymphocyte Activation / immunology Lymphocytes, Tumor-Infiltrating / immunology Male Mice Mice, Inbred BALB C Neoplasm Transplantation Spleen / cytology Spleen / immunology T-Lymphocytes, Cytotoxic / immunology Th1 Cells / immunology Th1 Cells / metabolism Transduction, Genetic |
| IF | 5.145 |
| 引用数 | 42 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | AxCAmIL12p40 Adex1CAmIL12p40 (RDB1417) AxCAmIL12p35 Adex1CAmIL12p35 (RDB1414) |