Reference - Detail
| RRC ID | 12165 |
|---|---|
| Author | Takekoshi K, Ishii K, Kawakami Y, Isobe K, Nakai T. |
| Title | kappa-Opioid inhibits catecholamine biosynthesis in PC12 rat pheochromocytoma cell. |
| Journal | FEBS Lett |
| Abstract |
It was reported that nicotine-induced dopamine release in the rat pheochromocytoma cell line, PC12 cells, was inhibited by kappa-opioid. However, it is not known whether inhibition of catecholamine biosynthesis is involved in the inhibitory mechanisms of kappa-opioids in PC12 cells. U-69593 (a kappa-opioid agonist: >/=100 nM) significantly inhibited the nicotine-induced increase of tyrosine hydroxylase (TH, a rate-limiting enzyme in biosynthesis of catecholamine) enzyme activity and TH mRNA levels. These inhibitory effects were completely reversed by naloxone and nor-binaltorphimine dihydrochloride (nor-BNI), a specific kappa-antagonist, whereas pertussis toxin (PTX) only partially reversed this inhibitory effect. Also, U-69593 (>/=100 nM) significantly inhibited the nicotine-induced increase of cAMP production. This inhibitory effect was completely reversed by naloxone and nor-BNI, whilst only partially reversed by PTX. Moreover, U-69593 (>/=100 nM) significantly inhibited the nicotine-induced increase of both the TH protein level and intracellular catecholamine levels. These results indicate that the anti-cholinergic actions of kappa-opioid can be explained partially by its inhibition of both TH enzyme activity and TH synthesis, through suppression of the cAMP/protein kinase A pathway. It would also appear that the PTX-sensitive G-protein mediates the inhibitory effect of this pathway, at least in part. |
| Volume | 477(3) |
| Pages | 273-7 |
| Published | 2000-7-21 |
| DOI | 10.1016/s0014-5793(00)01789-0 |
| PII | S0014-5793(00)01789-0 |
| PMID | 10908734 |
| MeSH | Animals Benzeneacetamides* Catecholamines / antagonists & inhibitors* Catecholamines / biosynthesis Cyclic AMP / biosynthesis Nicotine / pharmacology Opioid Peptides / pharmacology* PC12 Cells Pyrrolidines / pharmacology RNA, Messenger / genetics Rats Receptors, Opioid, kappa / agonists Tyrosine 3-Monooxygenase / antagonists & inhibitors Tyrosine 3-Monooxygenase / genetics |
| IF | 3.057 |
| Times Cited | 18 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pHTH1 Human tyrosine hydroxylase type I cDNA (RDB01269) |
| Human and Animal Cells | PC-12(RCB0009) |