論文 - 詳細
| RRC ID | 12169 |
|---|---|
| 著者 | Kurihara H, Zama A, Tamura M, Takeda J, Sasaki T, Takeuchi T. |
| タイトル | Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator. |
| ジャーナル | Gene Ther |
| Abstract |
For glioma- and glioblastoma-specific gene expression, we utilized a nestin regulatory element whose activity was evaluated by the reporter gene lacZ. Nestin is a 38-kDa intermediate filament protein, and is expressed specifically in the neuroepithelial stem cells. Nestin is detected in gliomas and glioblastomas, but not in normal brain tissue. We constructed a nestin gene regulator by placing nestin's second intron before the 5' upstream region (2iNP). To obtain enhanced expression of this tissue-specific regulator, we utilized the adenovirus double-infection method with a Cre-loxP on/off switching system. We constructed a 'regulator' vector, Ax2iNPNCre, which expresses Cre recombinase under the control of the nestin regulatory element, 2iNP. A 'reporter' vector, AxCALNLNZK, expresses lacZ under the control of a strong CAG promoter when the stuffer sequence has been removed by Cre recombinase at a pair of loxP sites. We used seven human glioma/glioblastoma cell lines: U251, KG-1C, NGM5, U87 MG, LN-Z308, NP-2 and T98G. Of these, nestin was expressed highly in U251 and KG-1C, less in NGM5, and undetectably in the other four lines. With the use of the two adenovirus vectors, we found X-gal staining and high nestin regulator-promoted beta-galactosidase activities in four of the seven glioma/glioblastoma cell lines. Staining was strong in U251, KG-1C and NGM5, and less in U87 MG. LacZ expression was nearly undetectable in the non-glioma cell line, HeLa, but a little in COS-7. The adenovirus double-infection method, which uses a nestin regulator, is applicable for glioma/glioblastoma-specific expression. |
| 巻・号 | 7(8) |
| ページ | 686-93 |
| 公開日 | 2000-4-1 |
| DOI | 10.1038/sj.gt.3301129 |
| PMID | 10800092 |
| MeSH | Adenoviridae / genetics* Brain Neoplasms / therapy* Gene Expression Genetic Therapy / methods* Genetic Vectors / administration & dosage* Glioblastoma / therapy Glioma / therapy* Humans Intermediate Filament Proteins / genetics* Lac Operon Nerve Tissue Proteins* Nestin Tumor Cells, Cultured |
| IF | 4.128 |
| 引用数 | 35 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pxCANCre (RDB01675) |
| ヒト・動物細胞 | KG-1-C(RCB0270) U251(RCB0461) |