RRC ID 12180
Author Kawada T, Ogasawara M, Sekiguchi T, Aoyama M, Hotta K, Oka K, Satake H.
Title Peptidomic analysis of the central nervous system of the protochordate, Ciona intestinalis: homologs and prototypes of vertebrate peptides and novel peptides.
Journal Endocrinology
Abstract The phylogenetic position of ascidians as the chordate invertebrates closest to vertebrates suggests that they might possess homologs and/or prototypes of vertebrate peptide hormones and neuropeptides as well as ascidian-specific peptides. However, only a small number of peptides have so far been identified in ascidians. In the present study, we have identified various peptides in the ascidian, Ciona intestinalis. Mass spectrometry-based peptidomic analysis detected 33 peptides, including 26 novel peptides, from C. intestinalis. The ascidian peptides are largely classified into three categories: 1) prototypes and homologs of vertebrate peptides, such as galanin/galanin-like peptide, which have never been identified in any invertebrates; 2) peptides partially homologous with vertebrate peptides, including novel neurotesin-like peptides; 3) novel peptides. These results not only provide evidence that C. intestinalis possesses various homologs and prototypes of vertebrate neuropeptides and peptide hormones but also suggest that several of these peptides might have diverged in the ascidian-specific evolutionary lineage. All Ciona peptide genes were expressed in the neural complex, whereas several peptide gene transcripts were also distributed in peripheral tissues, including the ovary. Furthermore, a Ciona neurotensin-like peptide, C. intestinalis neurotensin-like peptide 6, was shown to down-regulate growth of Ciona vitellogenic oocytes. These results suggest that the Ciona peptides act not only as neuropeptides in the neural tissue but also as hormones in nonneuronal tissues and that ascidians, unlike other invertebrates, such as nematodes, insects, and sea urchins, established an evolutionary origin of the peptidergic neuroendocrine, endocrine, and nervous systems of vertebrates with certain specific molecular diversity.
Volume 152(6)
Pages 2416-27
Published 2011-6
DOI 10.1210/en.2010-1348
PII en.2010-1348
PMID 21467196
MeSH Amino Acid Sequence Animals Central Nervous System / chemistry Central Nervous System / metabolism* Ciona intestinalis / chemistry Ciona intestinalis / genetics* Ciona intestinalis / metabolism Female Gene Expression Regulation Invertebrates / chemistry Invertebrates / classification Invertebrates / genetics Male Molecular Sequence Data Peptides / chemistry Peptides / genetics* Sequence Homology, Amino Acid Species Specificity Vertebrates / classification Vertebrates / genetics*
IF 3.961
Times Cited 16
WOS Category ENDOCRINOLOGY & METABOLISM
Resource
C.intestinalis / (O.japonicus) Wild C. int