論文 - 詳細
| RRC ID | 12182 |
|---|---|
| 著者 | Sakai T, Horii T, Hisaeda H, Zhang M, Ishii K, Nakano Y, Maekawa Y, Izumi K, Nitta Y, Miyazaki J, Himeno K. |
| タイトル | DNA immunization with Plasmodium falciparum serine repeat antigen: regulation of humoral immune response by coinoculation of cytokine expression plasmid. |
| ジャーナル | Parasitol Int |
| Abstract |
We immunized mice with plasmid expressing the 47-kDa amino-terminal domain of the Plasmodium falciparum serine repeat antigen (SERA) using gene gun and investigated humoral immune response to SERA antigen. Significant SERA-specific IgG was observed in BALB/c mice after immunization three times with SERA expression plasmid. Furthermore, these levels were increased by the coinoculation of cytokine (IFN-gamma, IL-4, GM-CSF, or IL-12) expression plasmid. In respect to the SERA-specific Ig subclasses, coinoculation of IFN-gamma, GM-CSF, or IL-12 expression plasmid increased the levels of SERA-specific IgG2a, and these were much higher than that in mice immunized with SERA expression plasmid alone. In contrast to the SERA-specific IgG2a, coinoculation of any cytokine expression plasmid did not change the levels of SERA-specific IgG1. These results indicate that cytokine expression plasmid enhances and regulates humoral immune response elicited by SERA DNA immunization. |
| 巻・号 | 48(1) |
| ページ | 27-33 |
| 公開日 | 1999-3-1 |
| DOI | 10.1016/s1383-5769(98)00036-1 |
| PII | S1383576998000361 |
| PMID | 11269323 |
| MeSH | Animals Antibodies, Protozoan / blood* Antigens, Protozoan / genetics Antigens, Protozoan / immunology* Biolistics / methods Cytokines / genetics Cytokines / immunology* Cytokines / metabolism Malaria Vaccines / genetics Malaria Vaccines / immunology* Malaria, Falciparum / prevention & control Mice Mice, Inbred BALB C Plasmids Plasmodium falciparum / immunology* Vaccination Vaccines, DNA / immunology* |
| IF | 1.866 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBluescript KS+mIFN gamma Mouse IFN gamma cDNA (RDB01482) pBluescript KS+mIL4 Mouse IL4 cDNA (RDB01473) pBluescript SKII+mGM-CSF Mouse GM-CSF cDNA (RDB01469) |