RRC ID |
12182
|
著者 |
Sakai T, Horii T, Hisaeda H, Zhang M, Ishii K, Nakano Y, Maekawa Y, Izumi K, Nitta Y, Miyazaki J, Himeno K.
|
タイトル |
DNA immunization with Plasmodium falciparum serine repeat antigen: regulation of humoral immune response by coinoculation of cytokine expression plasmid.
|
ジャーナル |
Parasitol Int
|
Abstract |
We immunized mice with plasmid expressing the 47-kDa amino-terminal domain of the Plasmodium falciparum serine repeat antigen (SERA) using gene gun and investigated humoral immune response to SERA antigen. Significant SERA-specific IgG was observed in BALB/c mice after immunization three times with SERA expression plasmid. Furthermore, these levels were increased by the coinoculation of cytokine (IFN-gamma, IL-4, GM-CSF, or IL-12) expression plasmid. In respect to the SERA-specific Ig subclasses, coinoculation of IFN-gamma, GM-CSF, or IL-12 expression plasmid increased the levels of SERA-specific IgG2a, and these were much higher than that in mice immunized with SERA expression plasmid alone. In contrast to the SERA-specific IgG2a, coinoculation of any cytokine expression plasmid did not change the levels of SERA-specific IgG1. These results indicate that cytokine expression plasmid enhances and regulates humoral immune response elicited by SERA DNA immunization.
|
巻・号 |
48(1)
|
ページ |
27-33
|
公開日 |
1999-3-1
|
DOI |
10.1016/s1383-5769(98)00036-1
|
PII |
S1383576998000361
|
PMID |
11269323
|
MeSH |
Animals
Antibodies, Protozoan / blood*
Antigens, Protozoan / genetics
Antigens, Protozoan / immunology*
Biolistics / methods
Cytokines / genetics
Cytokines / immunology*
Cytokines / metabolism
Malaria Vaccines / genetics
Malaria Vaccines / immunology*
Malaria, Falciparum / prevention & control
Mice
Mice, Inbred BALB C
Plasmids
Plasmodium falciparum / immunology*
Vaccination
Vaccines, DNA / immunology*
|
IF |
1.866
|
リソース情報 |
遺伝子材料 |
pBluescript KS+mIFN gamma Mouse IFN gamma cDNA (RDB01482)
pBluescript KS+mIL4 Mouse IL4 cDNA (RDB01473)
pBluescript SKII+mGM-CSF Mouse GM-CSF cDNA (RDB01469) |