| RRC ID |
12191
|
| Author |
Tatebe S, Matsuura T, Endo K, Teramachi K, Nakamura T, Sato K, Ito H.
|
| Title |
Adenovirus-mediated transfer of wild-type p53 gene results in apoptosis or growth arrest in human cultured gastric carcinoma cells.
|
| Journal |
Int J Oncol
|
| Abstract |
We examined the susceptibility of six human gastric carcinoma cell lines to infection with recombinant p53 adenovirus vector (AxCA-p53). AxCA-p53 infection at a muliplicity of infection (MOI) of 50 resulted in apoptotic cell death (MKN-1 cells), growth arrest (MKN-45, MKN-74 and KATO-III cells), or non-effectiveness (TMK-1 and OCUM-2M cells). Western blot analysis revealed increasing expression levels of p21/WAF1 protein after infection with AxCA-p53 in all the cell lines. After infection with AxCA-p53, the expression levels of bax or bcl-XL protein changed in MKN-1, but not in the other cell lines. These results suggest that the apoptotic pathway (dependence on the expressions of bcl-2 family proteins) dominates the growth arrest pathway (dependence on the expressions of p21/WAF1 protein) after infection with AxCA-p53. Thus, the bcl-2 family might play a crucial role in p53-mediated growth arrest and apoptosis in human gastric carcinoma cells.
|
| Volume |
15(2)
|
| Pages |
229-35
|
| Published |
1999-8-1
|
| DOI |
10.3892/ijo.15.2.229
|
| PMID |
10402231
|
| MeSH |
Adenoviridae / genetics*
Apoptosis / physiology*
Cell Cycle / physiology
Cell Division / physiology
DNA Fragmentation
Gene Transfer Techniques*
Genes, p53*
Genetic Vectors*
Humans
Proto-Oncogene Proteins c-bcl-2 / biosynthesis
Recombination, Genetic
Stomach Neoplasms / pathology
Stomach Neoplasms / therapy*
Tumor Cells, Cultured
|
| IF |
3.899
|
| Times Cited |
15
|
|
WOS Category
|
ONCOLOGY
|
| Resource |
| DNA material |
AxCALacZ Adex1CAlacZ (RDB02726) |
