論文 - 詳細
| RRC ID | 12205 |
|---|---|
| 著者 | Nishio Y, Kashiwagi A, Taki H, Shinozaki K, Maeno Y, Kojima H, Maegawa H, Haneda M, Hidaka H, Yasuda H, Horiike K, Kikkawa R. |
| タイトル | Altered activities of transcription factors and their related gene expression in cardiac tissues of diabetic rats. |
| ジャーナル | Diabetes |
| Abstract |
Gene regulation in the cardiovascular tissues of diabetic subjects has been reported to be altered. To examine abnormal activities in transcription factors as a possible cause of this altered gene regulation, we studied the activity of two redox-sensitive transcription factors--nuclear factor-kappaB (NF-kappaB) and activating protein-1 (AP-1)--and the change in the mRNA content of heme oxygenase-1, which is regulated by these transcription factors in the cardiac tissues of rats with streptozotocin-induced diabetes. Increased activity of NF-kappaB and AP-1 but not nuclear transcription-activating factor, as determined by an electrophoretic mobility shift assay, was found in the hearts of 4-week diabetic rats. Glycemic control by a subcutaneous injection of insulin prevented these diabetes-induced changes in transcription factor activity. In accordance with these changes, the mRNA content of heme oxygenase-1 was increased fourfold in 4-week diabetic rats and threefold in 24-week diabetic rats as compared with control rats (P < 0.01 and P < 0.05, respectively). Insulin treatment also consistently prevented changes in the mRNA content of heme oxygenase-1. The oral administration of an antioxidant, probucol, to these diabetic rats partially prevented the elevation of the activity of both NF-kappaB and AP-1, and normalized the mRNA content of heme oxygenase-1 without producing any change in the plasma glucose concentration. These results suggest that elevated oxidative stress is involved in the activation of the transcription factors NF-kappaB and AP-1 in the cardiac tissues of diabetic rats, and that these abnormal activities of transcription factors could be associated with the altered gene regulation observed in the cardiovascular tissues of diabetic rats. |
| 巻・号 | 47(8) |
| ページ | 1318-25 |
| 公開日 | 1998-8-1 |
| DOI | 10.2337/diab.47.8.1318 |
| PMID | 9703334 |
| MeSH | Animals Antioxidants / pharmacology Diabetes Mellitus, Experimental / genetics* Diabetes Mellitus, Experimental / metabolism* Gene Expression* / physiology Heart / physiopathology* Heme Oxygenase (Decyclizing) / genetics Heme Oxygenase-1 Lipid Peroxides / metabolism Male Myocardium / metabolism* Oxidoreductases / genetics Probucol / pharmacology RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Transcription Factors / genetics* Transcription Factors / physiology* |
| IF | 7.72 |
| 引用数 | 68 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pRHO1 Rat heme oxygenase cDNA (RDB01208) |