RRC ID 12227
Author Isobe K, Yukimasa N, Takekoshi K, Nomura F, Nakai T.
Title [Expression of mRNAs coding for catecholamine synthesizing enzymes in human adrenal pheochromocytoma].
Journal Rinsho Byori
Abstract Pheochromocytomas synthesize and release catecholamines, which subsequently are related to various clinical manifestations of the disease. However, pheochromocytomas are not innervated and the catecholamine release and synthesis are not initiated by neural impulses. It is still unknown how catecholamine synthesis is regulated in pheochromocytomas. As a first step toward understanding the molecular mechanisms by which catecholamine synthesis is controlled in the tumor, we measured the levels of mRNA coding for the catecholamine synthesizing enzyme, tyrosine hydroxylase (TH) and catecholamines in 6 pheochromocytomas and 2 normal adrenal glands. The TH mRNA level was overexpressed and the catecholamine contents were high in 4 out of 6 pheochromocytomas. There was a close correlation between the TH mRNA level and the catecholamines content in the tumors. We also examined the gene expression of the messengers of other catecholamine synthesizing enzymes, dopamine beta-hydroxylase (DBH) and aromatic 1-amino acid decarboxylase (AADC) in pheochromocytomas. The expression of these genes was in parallel with that of TH mRNA in the tumors. These findings indicate that catecholamine overproduction in pheochromocytomas is mediated by the overexpression of genes coding for catecholamines synthesizing enzymes, TH, DBH, and AADC.
Volume 43(6)
Pages 540-4
Published 1995-6-1
PMID 7602797
MeSH Adrenal Gland Neoplasms / enzymology* Adult Aged Catecholamines / biosynthesis* Female Humans Male Middle Aged Pheochromocytoma / enzymology* RNA, Messenger / metabolism* Tyrosine 3-Monooxygenase / genetics*
DNA material pDBH-1 (RDB01271) pPCDC13 (RDB01270) pHTH1 (RDB01269)