Reference - Detail
| RRC ID | 12251 |
|---|---|
| Author | Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando S, Sugiura M, Yoshida MA, Kasama Y, Abe M. |
| Title | Crucial role of c-Myc in the generation of induced pluripotent stem cells. |
| Journal | Stem Cells |
| Abstract |
c-Myc transduction has been considered previously to be nonessential for induced pluripotent stem cell (iPSC) generation. In this study, we investigated the effects of c-Myc transduction on the generation of iPSCs from an inbred mouse strain using a genome integration-free vector to exclude the effects of the genetic background and the genomic integration of exogenous genes. Our findings reveal a clear difference between iPSCs generated using the four defined factors including c-Myc (4F-iPSCs) and those produced without c-Myc (3F-iPSCs). Molecular and cellular analyses did not reveal any differences between 3F-iPSCs and 4F-iPSCs, as reported previously. However, a chimeric mice formation test indicated clear differences, whereby few highly chimeric mice and no germline transmission was observed using 3F-iPSCs. Similar differences were also observed in the mouse line that has been widely used in iPSC studies. Furthermore, the defect in 3F-iPSCs was considerably improved by trichostatin A, a histone deacetyl transferase inhibitor, indicating that c-Myc plays a crucial role in iPSC generation through the control of histone acetylation. Indeed, low levels of histone acetylation were observed in 3F-iPSCs. Our results shed new light on iPSC generation mechanisms and strongly recommend c-Myc transduction for preparing high-quality iPSCs. |
| Volume | 29(9) |
| Pages | 1362-70 |
| Published | 2011-9-1 |
| DOI | 10.1002/stem.685 |
| PMID | 21732496 |
| MeSH | Animals Blastomeres / physiology Chimera Embryonic Stem Cells / cytology Embryonic Stem Cells / physiology Female Genes, myc Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / metabolism Induced Pluripotent Stem Cells / physiology* Male Mice Mice, Inbred C57BL Pregnancy Proto-Oncogene Proteins c-myc / biosynthesis Proto-Oncogene Proteins c-myc / genetics* Transduction, Genetic |
| IF | 6.022 |
| Times Cited | 38 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ONCOLOGY HEMATOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Mice | RBRC02290 |