RRC ID 1229
著者 Ugai H, Watanabe S, Suzuki E, Tsutsui-Nakata H, Yokoyama KK, Murata T.
タイトル Stability of a recombinant adenoviral vector: optimization of conditions for storage, transport and delivery.
ジャーナル Jpn J Cancer Res
Abstract Recombinant viral vectors have been developed for use as therapeutic agents and for the introduction of exogenous genes into living cells. However, little is known about the viability and stability of such recombinant viruses during storage, transport and delivery under various conditions. We describe here an analysis of the stability of an adenoviral vector in crude solutions of cell lysates during freezing and thawing and during storage at various temperatures in the presence and in the absence of glycerol. For example, the titer of adenoviruses in crude lysates of infected cells was reduced only ten-fold or three-fold after two hundred rounds of freezing and thawing or after incubation at 28 degrees C for 14 days, respectively. Our observations indicate that recombinant adenoviral vector was more stable than expected both during freezing and thawing and during storage at low temperatures. Our results confirm the importance of appropriate conditions for the delivery and transport of recombinant adenoviral vectors.
巻・号 93(5)
ページ 598-603
公開日 2002-5-1
DOI 10.1111/j.1349-7006.2002.tb01296.x
PMID 12036457
PMC PMC5927025
MeSH Adenoviridae / genetics* Cell Line Freezing Gene Transfer Techniques* Genetic Therapy / methods* Genetic Vectors HeLa Cells Humans Specimen Handling Temperature Time Factors beta-Galactosidase / metabolism
引用数 13
WOS 分野 ONCOLOGY
リソース情報
遺伝子材料 AxCANLacZ (RDB01749)
ヒト・動物細胞 HeLa(RCB0007)