論文 - 詳細
| RRC ID | 12338 |
|---|---|
| 著者 | Miyazaki S, Katsumata T, Natsume M, Kawaide H. |
| タイトル | The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P. |
| ジャーナル | FEBS Lett |
| Abstract |
The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC(50) 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC(50) 0.26 μM). |
| 巻・号 | 585(12) |
| ページ | 1879-83 |
| 公開日 | 2011-6-23 |
| DOI | 10.1016/j.febslet.2011.04.057 |
| PII | S0014-5793(11)00316-4 |
| PMID | 21545802 |
| MeSH | Arabidopsis / enzymology Bryopsida / enzymology* Cytochrome P-450 Enzyme Inhibitors* Cytochrome P-450 Enzyme System Diterpenes / metabolism Diterpenes, Kaurane / metabolism Gibberellins / biosynthesis Plant Proteins Triazoles / pharmacology* |
| IF | 3.057 |
| 引用数 | 24 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdp63558 |