RRC ID 12338
Author Miyazaki S, Katsumata T, Natsume M, Kawaide H.
Title The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P.
Journal FEBS Lett.
Abstract The moss Physcomitrella patens produces both ent-kaurene and ent-kaurenoic acid, which are intermediates of gibberellin biosynthesis in flowering plants. The CYP701 superfamily of cytochrome P450s functions as ent-kaurene oxidases in the biosynthesis of ent-kaurenoic acid. A candidate gene encoding ent-kaurene oxidase in P. patens, CYP701B1, was cloned and heterologously expressed in yeast to examine enzyme activities in vitro. The recombinant CYP701B1 protein catalyzed the oxidation reaction from ent-kaurene to ent-kaurenoic acid. CYP701B1 activity was highly resistant to the ent-kaurene oxidase inhibitor uniconazole-P (IC(50) 64 μM), even though the activity of Arabidopsis ent-kaurene oxidase (CYP701A3) was sensitive (IC(50) 0.26 μM).
Volume 585(12)
Pages 1879-83
Published 2011-6-23
DOI 10.1016/j.febslet.2011.04.057
PII S0014-5793(11)00316-4
PMID 21545802
MeSH Arabidopsis / enzymology Bryopsida / enzymology* Cytochrome P-450 Enzyme Inhibitors* Cytochrome P-450 Enzyme System Diterpenes / metabolism Diterpenes, Kaurane / metabolism Gibberellins / biosynthesis Plant Proteins Triazoles / pharmacology*
IF 2.999
Times Cited 14
Arabidopsis / Cultured plant cells, genes pdp63558