Reference - Detail
| RRC ID | 1233 |
|---|---|
| Author | Muroi M, Tanamoto K. |
| Title | The polysaccharide portion plays an indispensable role in Salmonella lipopolysaccharide-induced activation of NF-kappaB through human toll-like receptor 4. |
| Journal | Infect Immun |
| Abstract |
The lipid A portion has been identified as the active center responsible for lipopolysaccharide (LPS)-induced macrophage activation. However, we found that Salmonella (Salmonella enterica serovars Abortusequi, Minnesota, and Typhimurium) lipid A is inactive in human macrophages, despite its LPS being highly active. Thus we investigated the critical role of polysaccharide in Salmonella LPS-induced activation of NF-kappaB. In human monocytic cell line THP-1, Salmonella lipid A and synthetic Salmonella-type lipid A (516) did not induce NF-kappaB-dependent reporter activity up to 1 micro g/ml, whereas strong activation was observed in response to Salmonella LPS. The difference in activity between this lipid A and LPS was further examined by using 293 cells expressing human CD14/Toll-like receptor 4 (TLR4)/MD-2, and similar results were obtained in these cells as well. A polysaccharide preparation obtained from Salmonella LPS was inactive in 293 cells expressing human CD14/TLR4/MD-2 even in combination with 516. Salmonella enterica serovar Minnesota Re LPS, whose structure consists of lipid A and two molecules of 2-keto-3-deoxyoctonic acid, but not its lipid A exhibited strong activity in THP-1 cells and 293 cells expressing human CD14/TLR4/MD-2. These results indicate that the polysaccharide portion covalently bound to lipid A plays the principal role in Salmonella LPS-induced activation of NF-kappaB through human CD14/TLR4/MD-2. |
| Volume | 70(11) |
| Pages | 6043-7 |
| Published | 2002-11-1 |
| DOI | 10.1128/IAI.70.11.6043-6047.2002 |
| PMID | 12379680 |
| PMC | PMC130318 |
| MeSH | Animals Antigens, Surface / physiology Cell Line Drosophila Proteins* Humans Lipid A / chemistry Lipid A / pharmacology* Lipopolysaccharide Receptors / physiology Lipopolysaccharides / chemistry Lipopolysaccharides / pharmacology* Lymphocyte Antigen 96 Membrane Glycoproteins / physiology* Mice NF-kappa B / metabolism* Polysaccharides, Bacterial / pharmacology* Receptors, Cell Surface / physiology* Salmonella / pathogenicity* Toll-Like Receptor 4 Toll-Like Receptors |
| IF | 3.201 |
| Times Cited | 60 |
| WOS Category | INFECTIOUS DISEASES IMMUNOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |