論文 - 詳細
| RRC ID | 1234 |
|---|---|
| 著者 | Ikeda M, Okai M, Miyoshi T, Tone S, Minatogawa Y. |
| タイトル | Transcriptional suppression of the estrogen receptor by truncated estrogen receptor-alpha. |
| ジャーナル | Horm Metab Res |
| Abstract |
The estrogen receptor (ER) is composed of six major functional domains - the A/B domain as the activation function 1 domain, domain C as the DNA-binding domain, domain D as a hinge domain, and domain E/F as the ligand-dependent transcriptional domains. A novel protein (designated as SRB-RGS) that interacted with domains C and D of ER alpha (ER alpha C/D) repressed the transcriptional activity of ER alpha. In this study, we have examined whether ER alpha C/D releases transcriptional suppression of ER alpha by intrinsic SRB-RGS. The expression vector of ER alpha C/D was transfected to the human cancer cell, KPL-1, which expressed the intrinsic ER. Unexpectedly, transcriptional suppression of ER by ER alpha C/D was observed. COS-7 cells, which have no intrinsic ER, showed a similar suppression of ER alpha by co-transfection of ER alpha and ER alpha C/D. The DNA-binding and the estrogen-binding activities of ER alpha decreased on co-transfection of ER alpha C/D, suggesting a decrease in the receptor protein itself. It is likely that the degradation of ER by co-transfection caused the transcriptional suppression of the ER. |
| 巻・号 | 34(8) |
| ページ | 425-30 |
| 公開日 | 2002-8-1 |
| DOI | 10.1055/s-2002-33599 |
| PMID | 12198596 |
| MeSH | Animals Breast Neoplasms / genetics Breast Neoplasms / metabolism COS Cells Chloramphenicol O-Acetyltransferase / genetics Chloramphenicol O-Acetyltransferase / metabolism Chlorocebus aethiops DNA / biosynthesis DNA / genetics Electrophoretic Mobility Shift Assay Estrogen Receptor alpha Humans Microscopy, Fluorescence Oligonucleotides Plasmids / genetics Protein Biosynthesis / genetics Rats Receptors, Estrogen / genetics* Suppression, Genetic / genetics Transcription, Genetic / genetics* Transfection Tumor Cells, Cultured |
| IF | 2.562 |
| 引用数 | 1 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |