| RRC ID |
12376
|
| Author |
Yamazoe H, Okuyama T, Suzuki H, Fukuda J.
|
| Title |
Fabrication of patterned cell co-cultures on albumin-based substrate: applications for microfluidic devices.
|
| Journal |
Acta Biomater
|
| Abstract |
A surface coated with cross-linked albumin film resists the adhesion of cells, and subsequent exposure to UV irradiation or electrostatic adsorption of a cationic polymer switches the surface from non-adherent to adherent. Taking advantage of this unique property of cross-linked albumin, the authors fabricated patterned cell co-cultures with desired patterns and cell types. In this scheme, the cell-adherent region was initially created in the cell-non-adhesive albumin substrate, on which a first cell type was attached. Subsequently, the remaining region was also changed to adherent for the attachment of secondary cells in the same manner, thereby allowing distinctly localized co-cultures. As a proof of concept demonstration of the feasibility of this approach, a patterned co-culture of Neuro-2a cells with L929 cells was successfully prepared on the substrate. Furthermore, combining this technique with a microfluidic technique, a micropatterned co-culture of PA6 cells with 3T3 fibroblasts was created inside microfluidic devices. This approach could potentially be a useful tool for fundamental investigations of cell-cell interactions and for tissue engineering applications.
|
| Volume |
6(2)
|
| Pages |
526-33
|
| Published |
2010-2-1
|
| DOI |
10.1016/j.actbio.2009.07.036
|
| PII |
S1742-7061(09)00321-3
|
| PMID |
19654059
|
| MeSH |
Albumins / metabolism*
Coculture Techniques
Humans
Microfluidics / instrumentation*
Microscopy, Electron
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
|
| IF |
7.242
|
| Times Cited |
16
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
L929(RCB1451)
MC3T3-G2/PA6RCB1127)
3T3 Swiss Albino(RCB1642) |
