| RRC ID |
12377
|
| Author |
Sakai S, Hirose K, Moriyama K, Kawakami K.
|
| Title |
Control of cellular adhesiveness in an alginate-based hydrogel by varying peroxidase and H(2)O(2) concentrations during gelation.
|
| Journal |
Acta Biomater
|
| Abstract |
An aqueous solution of alginate possessing phenolic hydroxyl (Alg-Ph) groups is gellable via a horseradish peroxidase (HRP)-catalyzed oxidative crosslinking reaction between Ph groups, consuming H(2)O(2) as an electron acceptor. This study evaluates the effect of H(2)O(2) and HRP concentrations on cellular adhesiveness and proliferation on the resultant enzymatically crosslinked Alg-Ph gels. After 4h of seeding, 81.1% of L929 fibroblast cells adhere to an Alg-Ph hydrogel prepared with 1 U ml(-1) HRP and 1mM H(2)O(2). Increasing the concentration of H(2)O(2) to 15 mM decreases the percentage of adhering cells to 28.4%. The cellular adhesion at this H(2)O(2) concentration is increased to 82.6% by increasing the HRP concentration to 10 U ml(-1). The cells adhering to the Alg-Ph hydrogels with higher cellular adhesiveness establish a confluent monolayer during 168 h of culture. A cell sheet can then be harvested within 5 min of immersion in a medium containing alginate lyase at 1.0 mg ml(-1). The harvested cell sheet re-adhere, and the cells contained in the sheet proliferate after being transferred to another cell culture dish.
|
| Volume |
6(4)
|
| Pages |
1446-52
|
| Published |
2010-4-1
|
| DOI |
10.1016/j.actbio.2009.10.004
|
| PII |
S1742-7061(09)00438-3
|
| PMID |
19818883
|
| MeSH |
Alginates / pharmacology*
Animals
Biocatalysis / drug effects
Cell Adhesion / drug effects
Cell Proliferation / drug effects
Fibroblasts / cytology
Fibroblasts / drug effects
Glucuronic Acid / pharmacology
Hexuronic Acids / pharmacology
Horseradish Peroxidase / pharmacology
Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate / pharmacology*
Hydrogen Peroxide / pharmacology*
Hydroxyl Radical
Mechanical Phenomena / drug effects
Mice
Solutions
Time Factors
|
| IF |
7.242
|
| Times Cited |
30
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
L929(RCB1451) |
