RRC ID 12382
Author Choi EM.
Title Modulatory effects of luteolin on osteoblastic function and inflammatory mediators in osteoblastic MC3T3-E1 cells.
Journal Cell Biol. Int.
Abstract The effects of luteolin on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts were investigated. Luteolin (1microM) caused a significant elevation of collagen content, alkaline phosphatase (ALP) activity, and osteocalcin secretion in the cells (P<0.05). The effect of luteolin in increasing collagen content and ALP activity was completely prevented by the presence of 10(-6)M cycloheximide and 10(-6)M tamoxifen, suggesting that luteolin's effect results from a newly synthesized protein component and might be partly involved in estrogen action. We then examined the effect of luteolin on the 3-morpholinosydnonimine (SIN-1)-induced production of oxidative stress markers [nitric oxide (NO) and prostaglan E(2) (PGE(2))] and cytokines [tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6)] in osteoblasts. Luteolin (1 and 10microM) decreased the SIN-1-induced production of NO, PGE(2), TNF-alpha, and IL-6 in osteoblasts. These results suggest that inflammatory mediators can be regulated by luteolin stimulating osteoblastic function.
Volume 31(9)
Pages 870-7
Published 2007-9
DOI 10.1016/j.cellbi.2007.01.038
PII S1065-6995(07)00053-4
PMID 17368935
MeSH Acetonitriles / pharmacology Alkaline Phosphatase / metabolism Animals Cell Line Collagen / biosynthesis Dinoprostone / biosynthesis Inflammation Mediators / metabolism* Interleukin-6 / biosynthesis Luteolin / chemistry Luteolin / pharmacology* Mice Morpholines / pharmacology Nitric Oxide / biosynthesis Osteoblasts / drug effects* Osteoblasts / metabolism* Osteocalcin / metabolism Phytoestrogens / chemistry Tumor Necrosis Factor-alpha / biosynthesis
IF 1.936
Times Cited 23
Human and Animal Cells