論文 - 詳細
| RRC ID | 12395 |
|---|---|
| 著者 | Okamoto H. |
| タイトル | Hepatitis E virus cell culture models. |
| ジャーナル | Virus Res |
| Abstract |
Early studies reported the propagation of hepatitis E virus (HEV) in either primary hepatocytes or several established cell lines, but replication was inefficient. Efficient cell culture systems for HEV in PLC/PRF/5 and A549 cells have recently been established, using inoculum of fecal suspensions with high HEV loads, originally obtained from patients with genotype 3 HEV (the JE03-1760F strain, 2.0×10(7) copies/ml) or genotype 4 HEV (the HE-JF5/15F strain, 1.3×10(7) copies/ml), and many generations were successfully propagated in serial passages of culture supernatant. In addition, a full-length infectious cDNA clone (pJE03-1760F/wt) of the JE03-1760F strain was constructed, which can replicate efficiently in PLC/PRF/5 and A549 cells. A derivative ORF3-deficient mutant revealed that the ORF3 protein of HEV is responsible for virion egress from infected cells and is present on the surface of released HEV particles, which is associated with lipids. Various HEV strains with high loads of ≥10(5) copies/ml in circulating blood were also propagated efficiently in PLC/PRF/5 and A549 cells. This paper reviews the road map toward the development of efficient cell culture systems for a wide variety of HEV strains and introduces the current knowledge on virion egress obtained by cell culture models. |
| 巻・号 | 161(1) |
| ページ | 65-77 |
| 公開日 | 2011-10-1 |
| DOI | 10.1016/j.virusres.2011.01.015 |
| PII | S0168-1702(11)00038-4 |
| PMID | 21316402 |
| MeSH | Animals Cell Line Hepatitis E / virology* Hepatitis E virus / genetics Hepatitis E virus / physiology* Humans Models, Biological Virus Cultivation |
| IF | 2.934 |
| 引用数 | 49 |
| WOS 分野 | VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |