論文 - 詳細
| RRC ID | 12397 |
|---|---|
| 著者 | Djalilian AR, Nagineni CN, Mahesh SP, Smith JA, Nussenblatt RB, Hooks JJ. |
| タイトル | Inhibition of inflammatory cytokine production in human corneal cells by dexamethasone, but not cyclosporin. |
| ジャーナル | Cornea |
| Abstract |
PURPOSE:To evaluate the modulatory effects of anti-inflammatory agents, dexamethasone (Dex) and cyclosporin A (CsA), on the production of cytokines and chemokines by human corneal cells in vitro following stimulation by the pro-inflammatory cytokine after interleukin 1beta (IL-1beta). METHODS:A human corneal epithelial (HCE) cell line and human corneal fibroblasts (HCFs) were stimulated in culture with IL-1beta and treated with Dex or CsA. The gene expression for selected cytokines and chemokines was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). The secretion of cytokines and chemokines was measured by enzyme-linked immunosorbent assay. RESULTS:IL-1beta enhanced the mRNA and/or protein levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-8, and monocyte chemotactic protein (MCP)-1 in HCE and IL-6, IL-8, MCP-3, and regulated on T-cell activation expressed secreted (RANTES) in HCFs. Treatment with CsA did not inhibit cytokine production in either HCE or HCFs. In contrast, Dex treatment inhibited the IL-1beta-induced production of GM-CSF, IL-6, IL-8, MCP-3, and RANTES, but not MCP-1. CONCLUSION:These results show that Dex, but not CsA, has direct immunosuppressive effects on the resident corneal cells, HCE and HCFs. This suggests that the clinically observed immunosuppressive effects of topical CsA are mediated primarily through the immune cells. |
| 巻・号 | 25(6) |
| ページ | 709-14 |
| 公開日 | 2006-7-1 |
| DOI | 10.1097/01.ico.0000208815.02120.90 |
| PII | 00003226-200607000-00015 |
| PMID | 17077666 |
| MeSH | Anti-Inflammatory Agents / pharmacology* Cell Culture Techniques Chemokine CCL2 / genetics Chemokine CCL5 / genetics Chemokine CCL7 Corneal Stroma / cytology Cyclosporine / pharmacology* Cytokines / genetics* Dexamethasone / pharmacology* Epithelium, Corneal / drug effects* Epithelium, Corneal / metabolism Fibroblasts / drug effects* Fibroblasts / metabolism Gene Expression / drug effects Granulocyte-Macrophage Colony-Stimulating Factor / genetics Humans Immunosuppressive Agents / pharmacology* Interleukin-1beta / pharmacology Interleukin-6 / genetics Interleukin-8 / genetics Monocyte Chemoattractant Proteins / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction |
| IF | 2.215 |
| 引用数 | 36 |
| WOS 分野 | OPHTHALMOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |