| RRC ID |
12407
|
| Author |
Okada Y, Okada T, Numata M, Hayashi Y, Yamashima T, Yamashita J.
|
| Title |
Increased expression of deoxyribonucleic acid methyltransferase gene in human astrocytic tumors.
|
| Journal |
Neurol Med Chir (Tokyo)
|
| Abstract |
The relationship between the grade of astrocytic tumor and the expression of deoxyribonucleic acid methyltransferase (DNA-MTase) gene was examined. The levels of DNA-MTase messenger ribonucleic acid (mRNA) were measured by semiquantitative reverse transcriptase-polymerase chain reaction in surgical specimens from 12 astrocytic tumors (4 astrocytomas, 6 anaplastic astrocytomas, and 2 glioblastomas) and two normal brain tissues, and in four glioma cell lines. Compared to normal brain tissues, the levels of DNA-MTase mRNA were increased by 16- to 55-fold in low grade astrocytomas, and significantly increased by 200- to 4500-fold in high grade astrocytomas (anaplastic astrocytomas and glioblastomas) and more than 4500-fold in glioma cell lines. In situ hybridization with paraffin-embedded surgical specimens of human astrocytic tumors showed DNA-MTase mRNA was abundantly expressed in high grade astrocytomas. The detection of increased DNA-MTase expression in astrocytic tumor indicates involvement in the tumorigenesis and suggests that blocking of this change with specific inhibitors may offer new therapeutic strategies for malignant astrocytic tumors.
|
| Volume |
40(11)
|
| Pages |
564-70; discussion 570-1
|
| Published |
2000-11-1
|
| DOI |
10.2176/nmc.40.564
|
| PII |
JST.JSTAGE/nmc/40.564
|
| PMID |
11109793
|
| MeSH |
Astrocytoma / genetics*
Blotting, Southern
Brain Neoplasms / genetics*
DNA, Neoplasm / genetics*
Gene Expression Regulation, Neoplastic
Glioblastoma / genetics
Humans
In Situ Hybridization
In Vitro Techniques
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
tRNA Methyltransferases / genetics*
|
| Times Cited |
17
|
|
WOS Category
|
RHEUMATOLOGY
|
| Resource |
| Human and Animal Cells |
U251(RCB0461)
U-87 MG(RCB0419) |
